Elongin functions as a loading factor for Mediator at ATF6α-regulated ER stress response genes

Yanfeng He; Shigeo Sato; Chieri Tomomori-Sato; Shiyuan Chen; Zach H. Goode; Joan W. Conaway; Ronald C. Conaway

doi:10.1073/pnas.2108751118

Elongin functions as a loading factor for Mediator at ATF6α-regulated ER stress response genes

Yanfeng He, Shigeo Sato, Chieri Tomomori-Sato, Shiyuan Chen, Zach H. Goode, Joan W. Conaway, Ronald C. Conaway

Research output: Contribution to journal › Article › peer-review

2 Scopus citations

Abstract

The bZIP transcription factor ATF6α is a master regulator of endoplasmic reticulum (ER) stress response genes. In this report, we identify the multifunctional RNA polymerase II transcription factor Elongin as a cofactor for ATF6α-dependent transcription activation. Biochemical studies reveal that Elongin functions at least in part by facilitating ATF6α-dependent loading of Mediator at the promoters and enhancers of ER stress response genes. Depletion of Elongin from cells leads to impaired transcription of ER stress response genes and to defects in the recruitment of Mediator and its CDK8 kinase subunit. Taken together, these findings bring to light a role for Elongin as a loading factor for Mediator during the ER stress response.

Original language	English (US)
Article number	e2108751118
Journal	Proceedings of the National Academy of Sciences of the United States of America
Volume	118
Issue number	39
DOIs	https://doi.org/10.1073/pnas.2108751118
State	Published - Sep 28 2021
Externally published	Yes

Keywords

Enhancer
Mediator
RNA polymerase II
Transcription

ASJC Scopus subject areas

General

Access to Document

10.1073/pnas.2108751118

Cite this

@article{ab6c6abd8f1047478059c4e3cef83d61,

title = "Elongin functions as a loading factor for Mediator at ATF6α-regulated ER stress response genes",

abstract = "The bZIP transcription factor ATF6α is a master regulator of endoplasmic reticulum (ER) stress response genes. In this report, we identify the multifunctional RNA polymerase II transcription factor Elongin as a cofactor for ATF6α-dependent transcription activation. Biochemical studies reveal that Elongin functions at least in part by facilitating ATF6α-dependent loading of Mediator at the promoters and enhancers of ER stress response genes. Depletion of Elongin from cells leads to impaired transcription of ER stress response genes and to defects in the recruitment of Mediator and its CDK8 kinase subunit. Taken together, these findings bring to light a role for Elongin as a loading factor for Mediator during the ER stress response.",

keywords = "Enhancer, Mediator, RNA polymerase II, Transcription",

author = "Yanfeng He and Shigeo Sato and Chieri Tomomori-Sato and Shiyuan Chen and Goode, {Zach H.} and Conaway, {Joan W.} and Conaway, {Ronald C.}",

year = "2021",

month = sep,

day = "28",

doi = "10.1073/pnas.2108751118",

language = "English (US)",

volume = "118",

journal = "Proceedings of the National Academy of Sciences of the United States of America",

issn = "0027-8424",

publisher = "National Academy of Sciences",

number = "39",

}

TY - JOUR

T1 - Elongin functions as a loading factor for Mediator at ATF6α-regulated ER stress response genes

AU - He, Yanfeng

AU - Sato, Shigeo

AU - Tomomori-Sato, Chieri

AU - Chen, Shiyuan

AU - Goode, Zach H.

AU - Conaway, Joan W.

AU - Conaway, Ronald C.

PY - 2021/9/28

Y1 - 2021/9/28

N2 - The bZIP transcription factor ATF6α is a master regulator of endoplasmic reticulum (ER) stress response genes. In this report, we identify the multifunctional RNA polymerase II transcription factor Elongin as a cofactor for ATF6α-dependent transcription activation. Biochemical studies reveal that Elongin functions at least in part by facilitating ATF6α-dependent loading of Mediator at the promoters and enhancers of ER stress response genes. Depletion of Elongin from cells leads to impaired transcription of ER stress response genes and to defects in the recruitment of Mediator and its CDK8 kinase subunit. Taken together, these findings bring to light a role for Elongin as a loading factor for Mediator during the ER stress response.

AB - The bZIP transcription factor ATF6α is a master regulator of endoplasmic reticulum (ER) stress response genes. In this report, we identify the multifunctional RNA polymerase II transcription factor Elongin as a cofactor for ATF6α-dependent transcription activation. Biochemical studies reveal that Elongin functions at least in part by facilitating ATF6α-dependent loading of Mediator at the promoters and enhancers of ER stress response genes. Depletion of Elongin from cells leads to impaired transcription of ER stress response genes and to defects in the recruitment of Mediator and its CDK8 kinase subunit. Taken together, these findings bring to light a role for Elongin as a loading factor for Mediator during the ER stress response.

KW - Enhancer

KW - Mediator

KW - RNA polymerase II

KW - Transcription

UR - http://www.scopus.com/inward/record.url?scp=85115325681&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85115325681&partnerID=8YFLogxK

U2 - 10.1073/pnas.2108751118

DO - 10.1073/pnas.2108751118

M3 - Article

C2 - 34544872

AN - SCOPUS:85115325681

SN - 0027-8424

VL - 118

JO - Proceedings of the National Academy of Sciences of the United States of America

JF - Proceedings of the National Academy of Sciences of the United States of America

IS - 39

M1 - e2108751118

ER -

Elongin functions as a loading factor for Mediator at ATF6α-regulated ER stress response genes

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this