Elongin (SIII): A multisubunit regulator of elongation by RNA polymerase II

Teijiro Aso, William S. Lane, Joan Weliky Conaway, Ronald C. Conaway

Research output: Contribution to journalArticlepeer-review

287 Scopus citations

Abstract

The Elongin (SIII) complex activates elongation by mammalian RNA polymerase II by suppressing transient pausing of the polymerase at many sites within transcription units. Elongin is a heterotrimer composed of A, B, and C subunits of 110, 18, and 15 kilodaltons, respectively. Here, the mammalian Elongin A gene was isolated and expressed, and the Elongin (SIII) complex reconstituted with recombinant subunits. Elongin A is shown to function as the transcriptionally active component of Elongin (SIII) and Elongin B and C as regulatory subunits. Whereas Elongin C assembles with Elongin A to form an AC complex with increased specific activity, Elongin B, a member of the ubiquitin-homology gene family, appears to serve a chaperone-like function, facilitating, assembly and enhancing stability of the Elongin (SIII) complex.

Original languageEnglish (US)
Pages (from-to)1439-1443
Number of pages5
JournalScience
Volume269
Issue number5229
DOIs
StatePublished - Sep 8 1995
Externally publishedYes

ASJC Scopus subject areas

  • General

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