TY - JOUR
T1 - Elongin (SIII)
T2 - A multisubunit regulator of elongation by RNA polymerase II
AU - Aso, Teijiro
AU - Lane, William S.
AU - Conaway, Joan Weliky
AU - Conaway, Ronald C.
PY - 1995/9/8
Y1 - 1995/9/8
N2 - The Elongin (SIII) complex activates elongation by mammalian RNA polymerase II by suppressing transient pausing of the polymerase at many sites within transcription units. Elongin is a heterotrimer composed of A, B, and C subunits of 110, 18, and 15 kilodaltons, respectively. Here, the mammalian Elongin A gene was isolated and expressed, and the Elongin (SIII) complex reconstituted with recombinant subunits. Elongin A is shown to function as the transcriptionally active component of Elongin (SIII) and Elongin B and C as regulatory subunits. Whereas Elongin C assembles with Elongin A to form an AC complex with increased specific activity, Elongin B, a member of the ubiquitin-homology gene family, appears to serve a chaperone-like function, facilitating, assembly and enhancing stability of the Elongin (SIII) complex.
AB - The Elongin (SIII) complex activates elongation by mammalian RNA polymerase II by suppressing transient pausing of the polymerase at many sites within transcription units. Elongin is a heterotrimer composed of A, B, and C subunits of 110, 18, and 15 kilodaltons, respectively. Here, the mammalian Elongin A gene was isolated and expressed, and the Elongin (SIII) complex reconstituted with recombinant subunits. Elongin A is shown to function as the transcriptionally active component of Elongin (SIII) and Elongin B and C as regulatory subunits. Whereas Elongin C assembles with Elongin A to form an AC complex with increased specific activity, Elongin B, a member of the ubiquitin-homology gene family, appears to serve a chaperone-like function, facilitating, assembly and enhancing stability of the Elongin (SIII) complex.
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U2 - 10.1126/science.7660129
DO - 10.1126/science.7660129
M3 - Article
C2 - 7660129
AN - SCOPUS:0029084914
SN - 0036-8075
VL - 269
SP - 1439
EP - 1443
JO - Science
JF - Science
IS - 5229
ER -