Embryonal carcinoma cells express Qa and Tla class I genes of the major histocompatibility complex

S. Ostrand-Rosenberg, D. A. Nickerson, V. K. Clements, E. P. Garcia, E. Lamouse-Smith, L. Hood, I. Stroynowski

Research output: Contribution to journalArticle

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Abstract

The murine major histocompatibility complex encodes H-2K and H-2D transplantation antigens and other class I-like proteins called Qa and Tla molecules; the functions of the Qa/Tla molecules are not known. That they may participate in embryonic cell-cell interactions and/or play a role in immune responses against tumors has been speculated. We have studied two murine embryonal carcinoma tumors, 402AX and PCC4, that are rejected in vivo immunologically, although they do not express H-2K or H-2D antigens. Transplantation studies with these cells suggest that rejection is mediated by class-I-like major histocompatibility complex antigens. As a first step in evaluating Qa/Tla function(s), we have characterized expression of class I-like genes and proteins in 402AX and PCC4 cells. Northern (RNA) blot hybridizations, polymerase chain reaction studies, and cDNA cloning experiments demonstrate that EC lines transcribe genes allelic to the Tla region gene '37', Qa-2 region gene 'Q7', and another, previously uncharacterized, class I-like gene. Immunoprecipitation studies show that the embryonal carcinoma tumor cells contain low levels of β2-microglobulin expressed in association with non-H-2K, non-H-2D class I-like proteins.

Original languageEnglish (US)
Pages (from-to)5084-5088
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume86
Issue number13
StatePublished - 1989

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MHC Class I Genes
Embryonal Carcinoma Stem Cells
Major Histocompatibility Complex
Histocompatibility Antigens
Embryonal Carcinoma
Genes
Neoplasms
Proteins
Immunoprecipitation
Cell Communication
Northern Blotting
Organism Cloning
Complementary DNA
Transplantation
RNA
Polymerase Chain Reaction

ASJC Scopus subject areas

  • General
  • Genetics

Cite this

Ostrand-Rosenberg, S., Nickerson, D. A., Clements, V. K., Garcia, E. P., Lamouse-Smith, E., Hood, L., & Stroynowski, I. (1989). Embryonal carcinoma cells express Qa and Tla class I genes of the major histocompatibility complex. Proceedings of the National Academy of Sciences of the United States of America, 86(13), 5084-5088.

Embryonal carcinoma cells express Qa and Tla class I genes of the major histocompatibility complex. / Ostrand-Rosenberg, S.; Nickerson, D. A.; Clements, V. K.; Garcia, E. P.; Lamouse-Smith, E.; Hood, L.; Stroynowski, I.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 86, No. 13, 1989, p. 5084-5088.

Research output: Contribution to journalArticle

Ostrand-Rosenberg, S, Nickerson, DA, Clements, VK, Garcia, EP, Lamouse-Smith, E, Hood, L & Stroynowski, I 1989, 'Embryonal carcinoma cells express Qa and Tla class I genes of the major histocompatibility complex', Proceedings of the National Academy of Sciences of the United States of America, vol. 86, no. 13, pp. 5084-5088.
Ostrand-Rosenberg, S. ; Nickerson, D. A. ; Clements, V. K. ; Garcia, E. P. ; Lamouse-Smith, E. ; Hood, L. ; Stroynowski, I. / Embryonal carcinoma cells express Qa and Tla class I genes of the major histocompatibility complex. In: Proceedings of the National Academy of Sciences of the United States of America. 1989 ; Vol. 86, No. 13. pp. 5084-5088.
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