Embryonic development of the ureter and bladder: Acquisition of smooth muscle

Linda A. Baker, R. Ariel Gomez

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

To delineate the temporal and spatial acquisition of the smooth muscle of the ureter, Sprague-Dawley rat embryos and newborn pups were immunostained with α-smooth muscle actin (α- SM actin) antibody, α-SM actin expression was first detected in the urinary tract at 16 days of gestation (E16) in a thin subserosal zone about the urogenital sinus. At this time, the E16 ureter is composed of a simple cuboidal epithelium which is surrounded by 1 to 2 layers of condensed α-SM actin negative spindle shaped cells. No immunostaining was detected along the ureter or its intrarenal branches until the 20th day of gestation (E20). α-SM actin expression in the E20 ureter exhibited regional differences. The number of α-SM actin positive smooth muscle ceils was greatest in the distal ureter, intermediate in the mid ureter, and least in the proximal ureter near the kidney. While smooth muscle formation in the bladder was subserosal, in the ureter it was subepithelial. During postnatal life, α-SM actin expression increased in both organs as all periepithelial spindle cells stained positive and intensified their staining. Smooth muscle differentiation of the ureter and bladder occurs later in embryonic life than other visceral and vascular organs and occurs in an ascending fashion from the bladder to the intrarenal collecting system. It is likely that the activation of visceral smooth muscle myogenesis within the urinary tract is governed by positional information specific to the embryonic development of each organ.

Original languageEnglish (US)
Pages (from-to)545-550
Number of pages6
JournalJournal of Urology
Volume160
Issue number2
DOIs
StatePublished - Aug 1998

Fingerprint

Ureter
Embryonic Development
Smooth Muscle
Urinary Bladder
Actins
Urinary Tract
Pregnancy
Muscle Development
Blood Vessels
Sprague Dawley Rats
Embryonic Structures
Epithelium
Staining and Labeling
Kidney
Antibodies

Keywords

  • Actins
  • Animals
  • Bladder
  • Fetal development
  • Immunohistochemistry
  • Newborn
  • Rats
  • S mooth muscle
  • Ureter

ASJC Scopus subject areas

  • Urology

Cite this

Embryonic development of the ureter and bladder : Acquisition of smooth muscle. / Baker, Linda A.; Gomez, R. Ariel.

In: Journal of Urology, Vol. 160, No. 2, 08.1998, p. 545-550.

Research output: Contribution to journalArticle

@article{13a51211b6ab49b3bd93893723d7fb96,
title = "Embryonic development of the ureter and bladder: Acquisition of smooth muscle",
abstract = "To delineate the temporal and spatial acquisition of the smooth muscle of the ureter, Sprague-Dawley rat embryos and newborn pups were immunostained with α-smooth muscle actin (α- SM actin) antibody, α-SM actin expression was first detected in the urinary tract at 16 days of gestation (E16) in a thin subserosal zone about the urogenital sinus. At this time, the E16 ureter is composed of a simple cuboidal epithelium which is surrounded by 1 to 2 layers of condensed α-SM actin negative spindle shaped cells. No immunostaining was detected along the ureter or its intrarenal branches until the 20th day of gestation (E20). α-SM actin expression in the E20 ureter exhibited regional differences. The number of α-SM actin positive smooth muscle ceils was greatest in the distal ureter, intermediate in the mid ureter, and least in the proximal ureter near the kidney. While smooth muscle formation in the bladder was subserosal, in the ureter it was subepithelial. During postnatal life, α-SM actin expression increased in both organs as all periepithelial spindle cells stained positive and intensified their staining. Smooth muscle differentiation of the ureter and bladder occurs later in embryonic life than other visceral and vascular organs and occurs in an ascending fashion from the bladder to the intrarenal collecting system. It is likely that the activation of visceral smooth muscle myogenesis within the urinary tract is governed by positional information specific to the embryonic development of each organ.",
keywords = "Actins, Animals, Bladder, Fetal development, Immunohistochemistry, Newborn, Rats, S mooth muscle, Ureter",
author = "Baker, {Linda A.} and Gomez, {R. Ariel}",
year = "1998",
month = "8",
doi = "10.1016/S0022-5347(01)62956-2",
language = "English (US)",
volume = "160",
pages = "545--550",
journal = "Journal of Urology",
issn = "0022-5347",
publisher = "Elsevier Inc.",
number = "2",

}

TY - JOUR

T1 - Embryonic development of the ureter and bladder

T2 - Acquisition of smooth muscle

AU - Baker, Linda A.

AU - Gomez, R. Ariel

PY - 1998/8

Y1 - 1998/8

N2 - To delineate the temporal and spatial acquisition of the smooth muscle of the ureter, Sprague-Dawley rat embryos and newborn pups were immunostained with α-smooth muscle actin (α- SM actin) antibody, α-SM actin expression was first detected in the urinary tract at 16 days of gestation (E16) in a thin subserosal zone about the urogenital sinus. At this time, the E16 ureter is composed of a simple cuboidal epithelium which is surrounded by 1 to 2 layers of condensed α-SM actin negative spindle shaped cells. No immunostaining was detected along the ureter or its intrarenal branches until the 20th day of gestation (E20). α-SM actin expression in the E20 ureter exhibited regional differences. The number of α-SM actin positive smooth muscle ceils was greatest in the distal ureter, intermediate in the mid ureter, and least in the proximal ureter near the kidney. While smooth muscle formation in the bladder was subserosal, in the ureter it was subepithelial. During postnatal life, α-SM actin expression increased in both organs as all periepithelial spindle cells stained positive and intensified their staining. Smooth muscle differentiation of the ureter and bladder occurs later in embryonic life than other visceral and vascular organs and occurs in an ascending fashion from the bladder to the intrarenal collecting system. It is likely that the activation of visceral smooth muscle myogenesis within the urinary tract is governed by positional information specific to the embryonic development of each organ.

AB - To delineate the temporal and spatial acquisition of the smooth muscle of the ureter, Sprague-Dawley rat embryos and newborn pups were immunostained with α-smooth muscle actin (α- SM actin) antibody, α-SM actin expression was first detected in the urinary tract at 16 days of gestation (E16) in a thin subserosal zone about the urogenital sinus. At this time, the E16 ureter is composed of a simple cuboidal epithelium which is surrounded by 1 to 2 layers of condensed α-SM actin negative spindle shaped cells. No immunostaining was detected along the ureter or its intrarenal branches until the 20th day of gestation (E20). α-SM actin expression in the E20 ureter exhibited regional differences. The number of α-SM actin positive smooth muscle ceils was greatest in the distal ureter, intermediate in the mid ureter, and least in the proximal ureter near the kidney. While smooth muscle formation in the bladder was subserosal, in the ureter it was subepithelial. During postnatal life, α-SM actin expression increased in both organs as all periepithelial spindle cells stained positive and intensified their staining. Smooth muscle differentiation of the ureter and bladder occurs later in embryonic life than other visceral and vascular organs and occurs in an ascending fashion from the bladder to the intrarenal collecting system. It is likely that the activation of visceral smooth muscle myogenesis within the urinary tract is governed by positional information specific to the embryonic development of each organ.

KW - Actins

KW - Animals

KW - Bladder

KW - Fetal development

KW - Immunohistochemistry

KW - Newborn

KW - Rats

KW - S mooth muscle

KW - Ureter

UR - http://www.scopus.com/inward/record.url?scp=0032322350&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0032322350&partnerID=8YFLogxK

U2 - 10.1016/S0022-5347(01)62956-2

DO - 10.1016/S0022-5347(01)62956-2

M3 - Article

C2 - 9679926

AN - SCOPUS:0032322350

VL - 160

SP - 545

EP - 550

JO - Journal of Urology

JF - Journal of Urology

SN - 0022-5347

IS - 2

ER -