Endocytic pathway is required for Drosophila Toll innate immune signaling

Hon Ren Huang, Zhijian J. Chen, Sam Kunes, Geen Dong Chang, Tom Maniatis

Research output: Contribution to journalArticle

60 Scopus citations


The Toll signaling pathway is required for the innate immune response against fungi and Gram-positive bacteria in Drosophila. Here we show that the endosomal proteins Myopic (Mop) and Hepatocyte growth factor-regulated tyrosine kinase substrate (Hrs) are required for the activation of the Toll signaling pathway. This requirement is observed in cultured cells and in flies, and epistasis experiments show that the Mop protein functions upstream of the MyD88 adaptor and the Pelle kinase. Mop and Hrs, which are critical components of the ESCRT-0 endocytosis complex, colocalize with the Toll receptor in endosomes. We conclude that endocytosis is required for the activation of the Toll signaling pathway.

Original languageEnglish (US)
Pages (from-to)8322-8327
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Issue number18
Publication statusPublished - May 4 2010


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