Endogenous angiotensin concentrations in specific intrarenal fluid compartments of the rat

Mouin G. Seikaly, Billy S. Arant, Frank D. Seney, Loyce Rutledge, Johnnie Green

Research output: Contribution to journalArticle

372 Citations (Scopus)

Abstract

To examine angiotensin (ANG) concentrations in fluid compartments near known intrarenal ANG receptors, we measured ANG concentrations in glomerular filtrate (GF), star vessel plasma (SVP), and luminal fluid from the early, mid, and late proximal tubule (E, M, and L PT). Samples were collected from euvolemic Munich-Wistar rats by free-flow micropuncture; ANG concentrations were measured by RIA. In one group of rats, concentrations of total immunoreactive ANG (reflecting ANG II and lesser amounts of three fragments) in GF and E, M, and L PT fluid averaged 29-40 nM compared with 32 pM in systemic plasma. In a second group, immunoreactive ANG concentrations in SVP also exceeded systemic levels by a factor of 1,000. In a final group, samples of GF and LPT fluid were purified by HPLC before RIA to measure ANG II and III concentrations specifically: their respective concentrations were 6-8 nM and 14-25 nM. We interpret these results to indicate that substantial amounts of ANG peptides are released into or generated within intrarenal fluid compartments, in which local ANG is likely to effect regulation of renal function independently of systemic ANG.

Original languageEnglish (US)
Pages (from-to)1352-1357
Number of pages6
JournalJournal of Clinical Investigation
Volume86
Issue number4
StatePublished - Oct 1990

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Angiotensins
Angiotensin II
Angiotensin III
Angiotensin Receptors
Punctures
Wistar Rats
High Pressure Liquid Chromatography
Kidney
Peptides

Keywords

  • Glomerulus
  • High performance liquid chromatography
  • Intrarenal angiotensin II
  • Proximal tubule
  • Radioimmunnoassay

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Endogenous angiotensin concentrations in specific intrarenal fluid compartments of the rat. / Seikaly, Mouin G.; Arant, Billy S.; Seney, Frank D.; Rutledge, Loyce; Green, Johnnie.

In: Journal of Clinical Investigation, Vol. 86, No. 4, 10.1990, p. 1352-1357.

Research output: Contribution to journalArticle

Seikaly, Mouin G. ; Arant, Billy S. ; Seney, Frank D. ; Rutledge, Loyce ; Green, Johnnie. / Endogenous angiotensin concentrations in specific intrarenal fluid compartments of the rat. In: Journal of Clinical Investigation. 1990 ; Vol. 86, No. 4. pp. 1352-1357.
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