Endogenous inhibitor of GABA binding in mammalian brain

Donald V. Greenlee, Paul C. van Ness, Richard W. Olsen

Research output: Contribution to journalArticle

105 Scopus citations

Abstract

Binding of radioactive gamma-aminobutyric acid to homogenates of mammalian brain was detected by a centrifugation assay. The binding capacity of the tissue was maximal and stable with time only if the tissue was thoroughly washed to remove an endogenous inhibitor of binding. With such washed tissue, binding to total rat or cow brain appeared to involve two populations of sites in the absence of sodium ions, the major site having a dissociation constant of 150 nM and saturating at 80 pmol/g brain, and a minor site with a KD of 20 nM and saturating at 20 pmol/g wet tissue. This sodium-independent GABA binding as a whole was localized in the crude mitochondrial, microsomal, and synaptosomal membrane fractions.

Original languageEnglish (US)
Pages (from-to)1653-1662
Number of pages10
JournalLife Sciences
Volume22
Issue number18
DOIs
StatePublished - May 8 1978

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Pharmacology, Toxicology and Pharmaceutics(all)

Fingerprint Dive into the research topics of 'Endogenous inhibitor of GABA binding in mammalian brain'. Together they form a unique fingerprint.

  • Cite this

    Greenlee, D. V., van Ness, P. C., & Olsen, R. W. (1978). Endogenous inhibitor of GABA binding in mammalian brain. Life Sciences, 22(18), 1653-1662. https://doi.org/10.1016/0024-3205(78)90062-0