Endogenous nitric oxide decreases xanthine oxidase-mediated neutrophil adherence: Role of P-selectin

Lance S. Terada, John E. Repine, Dale Piermattei, Brooks M. Hybertson

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

The oxygen radical-producing enzyme xanthine oxidase (XO) can promote neutrophil adherence to endothelium. Recognizing that a balance often exists in inflammatory processes, we sought to determine whether XO initiates antiadherent pathways. We found that bovine pulmonary arterial endothelial cells (EC) exposed to XO released increased amounts of nitrite into the media, reflecting an increased production of nitric oxide (NO). When EC were subjected to shear stress, treatment with XO and/or the NO synthase inhibitor N(ω)-nitro-L-arginine (L-NNA) increased neutrophil rolling behavior and firm neutrophil adherence to EC in an additive fashion. Both rolling and adherent interactions were abolished by monoclonal antibodies directed against P- selectin. In addition, treatment of EC with XO and/or L-NNA increased both surface expression of P-selectin and release of von Willebrand factor into media. Finally, treatment of EC with the NO donor sodium nitroprusside decreased XO-mediated neutrophil rolling and adherence. We conclude that XO stimulates EC to produce NO and that NO decreases the P-selectin-dependent neutrophil adhesion initiated by XO. Such increases in endogenous NO may constitute an important negative-feedback response to the acute proadhesive effects of XO.

Original languageEnglish (US)
Pages (from-to)913-917
Number of pages5
JournalJournal of Applied Physiology
Volume82
Issue number3
StatePublished - Mar 1997

Fingerprint

P-Selectin
Xanthine Oxidase
Nitric Oxide
Neutrophils
Endothelial Cells
Nitric Oxide Donors
von Willebrand Factor
Nitroprusside
Nitrites
Nitric Oxide Synthase
Endothelium
Arginine
Reactive Oxygen Species
Monoclonal Antibodies
Lung

Keywords

  • acute respiratory disease syndrome
  • endothelial cells
  • inflammation
  • multiple organ failure
  • neutrophils

ASJC Scopus subject areas

  • Physiology
  • Endocrinology
  • Orthopedics and Sports Medicine
  • Physical Therapy, Sports Therapy and Rehabilitation

Cite this

Endogenous nitric oxide decreases xanthine oxidase-mediated neutrophil adherence : Role of P-selectin. / Terada, Lance S.; Repine, John E.; Piermattei, Dale; Hybertson, Brooks M.

In: Journal of Applied Physiology, Vol. 82, No. 3, 03.1997, p. 913-917.

Research output: Contribution to journalArticle

Terada, Lance S. ; Repine, John E. ; Piermattei, Dale ; Hybertson, Brooks M. / Endogenous nitric oxide decreases xanthine oxidase-mediated neutrophil adherence : Role of P-selectin. In: Journal of Applied Physiology. 1997 ; Vol. 82, No. 3. pp. 913-917.
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