Endogenous production of angiotensin II modulates rat proximal tubule transport

Albert Quan, Michel Baum

Research output: Contribution to journalArticle

105 Scopus citations

Abstract

There is evidence that angiotensin II is synthesized by the proximal tubule and secreted into the tubular lumen. This study examined the functional significance of endogenously produced angiotensin Ii on proximal tubule transport in male Sprague-dawley rats. Addition of 10-11, 10-8, and 10-6M angiotensin II to the lumen of proximal convoluted tubules perfused in vivo had no effect on the rate of fluid reabsorption. The absence of an effect of exogenous luminal angiotensin II could be due to its endogenous production and luminal secretion. Luminal 10-8 M Dup 753 (an angiotensin II receptor antagonist) resulted in a 35% decrease in proximal tubule fluid reabsorption when compared to control (J(v) = 1.64±0.12 nl/mm · min vs. 2.55±0.32 nl/mm · min, P < 0.05). Similarly, luminal 10-4 M enalaprilat, an angiotensin converting enzyme inhibitor, decreased fluid reabsorption by 40% (J(v) = 1.53±0.23 nl/mm · min vs. 2.55±0.32 nl/mm · min, P < 0.05). When 10-11 or 10-8 M exogenous angiotensin II was added to enalaprilat (10-4 M) in the luminal perfusate, fluid reabsorption returned to its baseline rate (J(v) = 2.78±0.35 nl/mm · min). Thus, addition of exogenous angiotensin II stimulates proximal tubule transport when endogenous production is inhibited. These experiments show that endogenously produced angiotensin II modulates fluid transport in the proximal tubule independent of systemic angiotensin II.

Original languageEnglish (US)
Pages (from-to)2878-2882
Number of pages5
JournalJournal of Clinical Investigation
Volume97
Issue number12
DOIs
StatePublished - Jun 15 1996

Keywords

  • autocrine
  • enalaprilat
  • kidney
  • microperfusion
  • renin

ASJC Scopus subject areas

  • Medicine(all)

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