Endolysosomal two-pore channels regulate autophagy in cardiomyocytes

Vanessa García-Rúa, Sandra Feijóo-Bandín, Diego Rodríguez-Penas, Ana Mosquera-Leal, Emad Abu-Assi, Andrés Beiras, Luisa María Seoane, Pamela Lear, John Parrington, Manuel Portolés, Esther Roselló-Lletí, Miguel Rivera, Oreste Gualillo, Valentina Parra, Joseph A Hill, Beverly A Rothermel, José Ramón González-Juanatey, Francisca Lago

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Key points: Two-pore channels (TPCs) were identified as a novel family of endolysosome-targeted calcium release channels gated by nicotinic acid adenine dinucleotide phosphate, as also as intracellular Na+ channels able to control endolysosomal fusion, a key process in autophagic flux. Autophagy, an evolutionarily ancient response to cellular stress, has been implicated in the pathogenesis of a wide range of cardiovascular pathologies, including heart failure. We report direct evidence indicating that TPCs are involved in regulating autophagy in cardiomyocytes, and that TPC knockout mice show alterations in the cardiac lysosomal system. TPC downregulation implies a decrease in the viability of cardiomyocytes under starvation conditions. In cardiac tissues from both humans and rats, TPC transcripts and protein levels were higher in females than in males, and correlated negatively with markers of autophagy. We conclude that the endolysosomal channels TPC1 and TPC2 are essential for appropriate basal and induced autophagic flux in cardiomyocytes, and also that they are differentially expressed in male and female hearts. Autophagy participates in physiological and pathological remodelling of the heart. The endolysosomal two-pore channels (TPCs), TPC1 and TPC2, have been implicated in the regulation of autophagy. The present study aimed to investigate the role of TPC1 and TPC2 in basal and induced cardiac autophagic activity. In cultured cardiomyocytes, starvation induced a significant increase in TPC1 and TPC2 transcripts and protein levels that paralleled the increase in autophagy identified by increased LC3-II and decreased p62 levels. Small interfering RNA depletion of TPC2 alone or together with TPC1 increased both LC3II and p62 levels under basal conditions and in response to serum starvation, suggesting that, under conditions of severe energy depletion (serum plus glucose starvation), changes in the autophagic flux (as assessed by use of bafilomycin A1) occurred either when TPC1 or TPC2 were downregulated. The knockdown of TPCs diminished cardiomyocyte viability under starvation and simulated ischaemia. Electron micrographs of hearts from TPC1/2 double knockout mice showed that cardiomyocytes contained large numbers of immature lysosomes with diameters significantly smaller than those of wild-type mice. In cardiac tissues from humans and rats, TPC1 and TPC2 transcripts and protein levels were higher in females than in males. Furthermore, transcript levels of TPCs correlated negatively with p62 levels in heart tissues. TPC1 and TPC2 are essential for appropriate basal and induced autophagic flux in cardiomyocytes (i.e. there is a negative effect on cell viability under stress conditions in their absence) and they are differentially expressed in male and female human and murine hearts, where they correlate with markers of autophagy.

Original languageEnglish (US)
JournalJournal of Physiology
DOIs
StateAccepted/In press - 2016

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Autophagy
Cardiac Myocytes
Starvation
Knockout Mice
Down-Regulation
Proteins
Calcium Channels
Lysosomes
Serum
Small Interfering RNA
Cell Survival
Ischemia
Heart Failure
Electrons
Pathology
Glucose

ASJC Scopus subject areas

  • Physiology

Cite this

García-Rúa, V., Feijóo-Bandín, S., Rodríguez-Penas, D., Mosquera-Leal, A., Abu-Assi, E., Beiras, A., ... Lago, F. (Accepted/In press). Endolysosomal two-pore channels regulate autophagy in cardiomyocytes. Journal of Physiology. https://doi.org/10.1113/JP271332

Endolysosomal two-pore channels regulate autophagy in cardiomyocytes. / García-Rúa, Vanessa; Feijóo-Bandín, Sandra; Rodríguez-Penas, Diego; Mosquera-Leal, Ana; Abu-Assi, Emad; Beiras, Andrés; María Seoane, Luisa; Lear, Pamela; Parrington, John; Portolés, Manuel; Roselló-Lletí, Esther; Rivera, Miguel; Gualillo, Oreste; Parra, Valentina; Hill, Joseph A; Rothermel, Beverly A; González-Juanatey, José Ramón; Lago, Francisca.

In: Journal of Physiology, 2016.

Research output: Contribution to journalArticle

García-Rúa, V, Feijóo-Bandín, S, Rodríguez-Penas, D, Mosquera-Leal, A, Abu-Assi, E, Beiras, A, María Seoane, L, Lear, P, Parrington, J, Portolés, M, Roselló-Lletí, E, Rivera, M, Gualillo, O, Parra, V, Hill, JA, Rothermel, BA, González-Juanatey, JR & Lago, F 2016, 'Endolysosomal two-pore channels regulate autophagy in cardiomyocytes', Journal of Physiology. https://doi.org/10.1113/JP271332
García-Rúa V, Feijóo-Bandín S, Rodríguez-Penas D, Mosquera-Leal A, Abu-Assi E, Beiras A et al. Endolysosomal two-pore channels regulate autophagy in cardiomyocytes. Journal of Physiology. 2016. https://doi.org/10.1113/JP271332
García-Rúa, Vanessa ; Feijóo-Bandín, Sandra ; Rodríguez-Penas, Diego ; Mosquera-Leal, Ana ; Abu-Assi, Emad ; Beiras, Andrés ; María Seoane, Luisa ; Lear, Pamela ; Parrington, John ; Portolés, Manuel ; Roselló-Lletí, Esther ; Rivera, Miguel ; Gualillo, Oreste ; Parra, Valentina ; Hill, Joseph A ; Rothermel, Beverly A ; González-Juanatey, José Ramón ; Lago, Francisca. / Endolysosomal two-pore channels regulate autophagy in cardiomyocytes. In: Journal of Physiology. 2016.
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T1 - Endolysosomal two-pore channels regulate autophagy in cardiomyocytes

AU - García-Rúa, Vanessa

AU - Feijóo-Bandín, Sandra

AU - Rodríguez-Penas, Diego

AU - Mosquera-Leal, Ana

AU - Abu-Assi, Emad

AU - Beiras, Andrés

AU - María Seoane, Luisa

AU - Lear, Pamela

AU - Parrington, John

AU - Portolés, Manuel

AU - Roselló-Lletí, Esther

AU - Rivera, Miguel

AU - Gualillo, Oreste

AU - Parra, Valentina

AU - Hill, Joseph A

AU - Rothermel, Beverly A

AU - González-Juanatey, José Ramón

AU - Lago, Francisca

PY - 2016

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N2 - Key points: Two-pore channels (TPCs) were identified as a novel family of endolysosome-targeted calcium release channels gated by nicotinic acid adenine dinucleotide phosphate, as also as intracellular Na+ channels able to control endolysosomal fusion, a key process in autophagic flux. Autophagy, an evolutionarily ancient response to cellular stress, has been implicated in the pathogenesis of a wide range of cardiovascular pathologies, including heart failure. We report direct evidence indicating that TPCs are involved in regulating autophagy in cardiomyocytes, and that TPC knockout mice show alterations in the cardiac lysosomal system. TPC downregulation implies a decrease in the viability of cardiomyocytes under starvation conditions. In cardiac tissues from both humans and rats, TPC transcripts and protein levels were higher in females than in males, and correlated negatively with markers of autophagy. We conclude that the endolysosomal channels TPC1 and TPC2 are essential for appropriate basal and induced autophagic flux in cardiomyocytes, and also that they are differentially expressed in male and female hearts. Autophagy participates in physiological and pathological remodelling of the heart. The endolysosomal two-pore channels (TPCs), TPC1 and TPC2, have been implicated in the regulation of autophagy. The present study aimed to investigate the role of TPC1 and TPC2 in basal and induced cardiac autophagic activity. In cultured cardiomyocytes, starvation induced a significant increase in TPC1 and TPC2 transcripts and protein levels that paralleled the increase in autophagy identified by increased LC3-II and decreased p62 levels. Small interfering RNA depletion of TPC2 alone or together with TPC1 increased both LC3II and p62 levels under basal conditions and in response to serum starvation, suggesting that, under conditions of severe energy depletion (serum plus glucose starvation), changes in the autophagic flux (as assessed by use of bafilomycin A1) occurred either when TPC1 or TPC2 were downregulated. The knockdown of TPCs diminished cardiomyocyte viability under starvation and simulated ischaemia. Electron micrographs of hearts from TPC1/2 double knockout mice showed that cardiomyocytes contained large numbers of immature lysosomes with diameters significantly smaller than those of wild-type mice. In cardiac tissues from humans and rats, TPC1 and TPC2 transcripts and protein levels were higher in females than in males. Furthermore, transcript levels of TPCs correlated negatively with p62 levels in heart tissues. TPC1 and TPC2 are essential for appropriate basal and induced autophagic flux in cardiomyocytes (i.e. there is a negative effect on cell viability under stress conditions in their absence) and they are differentially expressed in male and female human and murine hearts, where they correlate with markers of autophagy.

AB - Key points: Two-pore channels (TPCs) were identified as a novel family of endolysosome-targeted calcium release channels gated by nicotinic acid adenine dinucleotide phosphate, as also as intracellular Na+ channels able to control endolysosomal fusion, a key process in autophagic flux. Autophagy, an evolutionarily ancient response to cellular stress, has been implicated in the pathogenesis of a wide range of cardiovascular pathologies, including heart failure. We report direct evidence indicating that TPCs are involved in regulating autophagy in cardiomyocytes, and that TPC knockout mice show alterations in the cardiac lysosomal system. TPC downregulation implies a decrease in the viability of cardiomyocytes under starvation conditions. In cardiac tissues from both humans and rats, TPC transcripts and protein levels were higher in females than in males, and correlated negatively with markers of autophagy. We conclude that the endolysosomal channels TPC1 and TPC2 are essential for appropriate basal and induced autophagic flux in cardiomyocytes, and also that they are differentially expressed in male and female hearts. Autophagy participates in physiological and pathological remodelling of the heart. The endolysosomal two-pore channels (TPCs), TPC1 and TPC2, have been implicated in the regulation of autophagy. The present study aimed to investigate the role of TPC1 and TPC2 in basal and induced cardiac autophagic activity. In cultured cardiomyocytes, starvation induced a significant increase in TPC1 and TPC2 transcripts and protein levels that paralleled the increase in autophagy identified by increased LC3-II and decreased p62 levels. Small interfering RNA depletion of TPC2 alone or together with TPC1 increased both LC3II and p62 levels under basal conditions and in response to serum starvation, suggesting that, under conditions of severe energy depletion (serum plus glucose starvation), changes in the autophagic flux (as assessed by use of bafilomycin A1) occurred either when TPC1 or TPC2 were downregulated. The knockdown of TPCs diminished cardiomyocyte viability under starvation and simulated ischaemia. Electron micrographs of hearts from TPC1/2 double knockout mice showed that cardiomyocytes contained large numbers of immature lysosomes with diameters significantly smaller than those of wild-type mice. In cardiac tissues from humans and rats, TPC1 and TPC2 transcripts and protein levels were higher in females than in males. Furthermore, transcript levels of TPCs correlated negatively with p62 levels in heart tissues. TPC1 and TPC2 are essential for appropriate basal and induced autophagic flux in cardiomyocytes (i.e. there is a negative effect on cell viability under stress conditions in their absence) and they are differentially expressed in male and female human and murine hearts, where they correlate with markers of autophagy.

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