Endothelial and perivascular cells maintain haematopoietic stem cells

Lei Ding, Thomas L. Saunders, Grigori Enikolopov, Sean J. Morrison

Research output: Contribution to journalArticle

970 Scopus citations

Abstract

Several cell types have been proposed to create niches for haematopoietic stem cells (HSCs). However, the expression patterns of HSC maintenance factors have not been systematically studied and no such factor has been conditionally deleted from any candidate niche cell. Thus, the cellular sources of these factors are undetermined. Stem cell factor (SCF; also known as KITL) is a key niche component that maintains HSCs. Here, using Scfgfp knock-in mice, we found that Scf was primarily expressed by perivascular cells throughout the bone marrow. HSC frequency and function were not affected when Scf was conditionally deleted from haematopoietic cells, osteoblasts, nestin-cre-or nestin-creER-expressing cells. However, HSCs were depleted from bone marrow when Scf was deleted from endothelial cells or leptin receptor (Lepr)-expressing perivascular stromal cells. Most HSCs were lost when Scf was deleted from both endothelial and Lepr-expressing perivascular cells. Thus, HSCs reside in a perivascular niche in which multiple cell types express factors that promote HSC maintenance.

Original languageEnglish (US)
Pages (from-to)457-462
Number of pages6
JournalNature
Volume481
Issue number7382
DOIs
StatePublished - Jan 26 2012

ASJC Scopus subject areas

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