Endothelial cell binding to dacron modified with polyethylene oxide and peptide

D. B. Holt, R. C. Eberhart, M. D. Prager

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Polyethylene oxide (PEO) was incorporated into the surface of Dacron (PET) vascular prosthetic material (crimped Bionit I, BNI) followed by covalent attachment of an endothelial cell (EC) adhesion peptide: Gly-Arg-Glu-Asp- Val-Tyr (GREDVY). This procedure provides the possibility of a surface selective for EC adherence. Optimal PEO incorporation with minimal fiber damage was achieved from 78% (vol) trifluoroacetic acid (TFA) as characterized by scanning electron microscopy, nuclear magnetic resonance, bromphenol blue staining, and tensile testing. By weight, there was 4.4 times as much 18.5kD PEO as 1.5kD PEO incorporated into PET, but there were 2.8 times as many molecules of low molecular weight PEO. Attachment of 125I- GREDVY to substrates increased as follows: BNI < BNI - 18.5 kD PEO < BNI - 1.5 kD PEO. Polyethylene oxide size affected EC binding with high molecular weight material decreasing binding and low molecular weight PEO increasing attachment. GREDVY modification of BNI or either of the two BNI-PEOs gave small but consistently increased EC binding compared with the same preparation without GREDVY. In contrast, human fibroblasts cultured from umbilical vein showed decreased binding when GREDVY was attached to BNI or PEO modified BNI. These results indicate that selective EC binding to PET vascular prostheses can be acheived.

Original languageEnglish (US)
JournalASAIO Journal
Volume40
Issue number3
StatePublished - Jul 1994

Fingerprint

Polyethylene Terephthalates
Endothelial cells
Polyethylene oxides
Peptides
Endothelial Cells
Molecular Weight
Molecular weight
Prosthetics
Bromphenol Blue
Blood Vessel Prosthesis
Trifluoroacetic Acid
Umbilical Veins
Trifluoroacetic acid
Cell adhesion
Tensile testing
Fibroblasts
Cell Adhesion
Electron Scanning Microscopy
Blood Vessels
Magnetic Resonance Spectroscopy

ASJC Scopus subject areas

  • Biophysics
  • Bioengineering

Cite this

Holt, D. B., Eberhart, R. C., & Prager, M. D. (1994). Endothelial cell binding to dacron modified with polyethylene oxide and peptide. ASAIO Journal, 40(3).

Endothelial cell binding to dacron modified with polyethylene oxide and peptide. / Holt, D. B.; Eberhart, R. C.; Prager, M. D.

In: ASAIO Journal, Vol. 40, No. 3, 07.1994.

Research output: Contribution to journalArticle

Holt, DB, Eberhart, RC & Prager, MD 1994, 'Endothelial cell binding to dacron modified with polyethylene oxide and peptide', ASAIO Journal, vol. 40, no. 3.
Holt, D. B. ; Eberhart, R. C. ; Prager, M. D. / Endothelial cell binding to dacron modified with polyethylene oxide and peptide. In: ASAIO Journal. 1994 ; Vol. 40, No. 3.
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