Endothelial cells are part of the normal bone marrow stroma. We have previously shown human umbilical cord blood [UCB) does not produce stroma in standard long-term cultures. Highly enriched (93-98%) UCB CD34+ cells were cultured for 6 weeks with interleukin-2 and conditioned medium from the 5637 carcinoma cell line (n = 4). The resulting 'fibroblast like' cells were shown to be endothelial by expression of von Willebrand factor (VWF), ICAM-1 (CD54), E-selectin (CD62(E)) and PECAM (CD31). Endothelial monolayers seeded with CD34+ UCB cells supported expansion of colony forming cells and CD34+ cells. We conclude that endothelial cell precursors circulate in UCB, and may be derived from the CD34+ cell fraction.
|Original language||English (US)|
|Number of pages||3|
|Journal||British Journal of Haematology|
|State||Published - 1997|
- Endothelial cell
- Umbilical cord blood
ASJC Scopus subject areas