Endothelial cell precursors are normal components of human umbilical cord blood

Mie Nieda, Andrew Nicol, Patricia Denning-Kendall, John Sweetenham, Ben Bradley, Jill Hows

Research output: Contribution to journalArticle

128 Scopus citations

Abstract

Endothelial cells are part of the normal bone marrow stroma. We have previously shown human umbilical cord blood [UCB) does not produce stroma in standard long-term cultures. Highly enriched (93-98%) UCB CD34+ cells were cultured for 6 weeks with interleukin-2 and conditioned medium from the 5637 carcinoma cell line (n = 4). The resulting 'fibroblast like' cells were shown to be endothelial by expression of von Willebrand factor (VWF), ICAM-1 (CD54), E-selectin (CD62(E)) and PECAM (CD31). Endothelial monolayers seeded with CD34+ UCB cells supported expansion of colony forming cells and CD34+ cells. We conclude that endothelial cell precursors circulate in UCB, and may be derived from the CD34+ cell fraction.

Original languageEnglish (US)
Pages (from-to)775-777
Number of pages3
JournalBritish Journal of Haematology
Volume98
Issue number3
DOIs
StatePublished - Jan 1 1997
Externally publishedYes

Keywords

  • CD34
  • Endothelial cell
  • Fibroblast
  • Macrophage
  • Umbilical cord blood

ASJC Scopus subject areas

  • Hematology

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