Endothelial ischemia-reperfusion injury in humans: Association with age and habitual exercise

Allison E. DeVan, Daniel Umpierre, Michelle L. Harrison, Hsin Fu Lin, Takashi Tarumi, Christopher P. Renzi, Mandeep Dhindsa, Stacy D. Hunter, Hirofumi Tanaka

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

Advancing age is a major risk factor for coronary artery disease. Endothelial dysfunction accompanied by increased oxidative stress and inflammation with aging may predispose older arteries to greater ischemia-reperfusion (I/R) injury. Because coronary artery ischemia cannot be induced safely, the effects of age and habitual endurance exercise on endothelial I/R injury have not been determined in humans. Using the brachial artery as a surrogate model of the coronary arteries, endothelial function, assessed by brachial artery flow-mediated dilation (FMD), was measured before and after 20 min of continuous forearm occlusion in young sedentary (n = 10, 24 ± 2 yr) and middle-aged (n = 9, 48 ± 2 yr) sedentary adults to gain insight into the effects of primary aging on endothelial I/R injury. Young (n = 9, 25 ± 1 yr) and middle-aged endurance-trained (n = 9, 50 ± 2 yr) adults were also studied to determine whether habitual exercise provides protection from I/R injury. Fifteen minutes after ischemic injury, FMD decreased significantly by 37% in young sedentary, 35% in young endurancetrained, 68% in middle-aged sedentary, and 50% in middle-aged endurance-trained subjects. FMD returned to baseline levels within 30 min in young sedentary and endurance-trained subjects but remained depressed in middle-aged sedentary and endurance-trained subjects. Circulating markers of antioxidant capacity and inflammation were not related to FMD. In conclusion, advancing age is associated with a greater magnitude and delayed recovery from endothelial I/R injury in humans. Habitual endurance exercise may provide partial protection to the endothelium against this form of I/R injury with advancing age.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Heart and Circulatory Physiology
Volume300
Issue number3
DOIs
StatePublished - Mar 1 2011

Fingerprint

Reperfusion Injury
Dilatation
Brachial Artery
Coronary Vessels
Inflammation
Forearm
Endothelium
Coronary Artery Disease
Oxidative Stress
Ischemia
Arteries
Antioxidants
Wounds and Injuries

Keywords

  • Brachial artery
  • Endothelium
  • Flow-mediated dilation

ASJC Scopus subject areas

  • Physiology
  • Physiology (medical)
  • Cardiology and Cardiovascular Medicine

Cite this

Endothelial ischemia-reperfusion injury in humans : Association with age and habitual exercise. / DeVan, Allison E.; Umpierre, Daniel; Harrison, Michelle L.; Lin, Hsin Fu; Tarumi, Takashi; Renzi, Christopher P.; Dhindsa, Mandeep; Hunter, Stacy D.; Tanaka, Hirofumi.

In: American Journal of Physiology - Heart and Circulatory Physiology, Vol. 300, No. 3, 01.03.2011.

Research output: Contribution to journalArticle

DeVan, Allison E. ; Umpierre, Daniel ; Harrison, Michelle L. ; Lin, Hsin Fu ; Tarumi, Takashi ; Renzi, Christopher P. ; Dhindsa, Mandeep ; Hunter, Stacy D. ; Tanaka, Hirofumi. / Endothelial ischemia-reperfusion injury in humans : Association with age and habitual exercise. In: American Journal of Physiology - Heart and Circulatory Physiology. 2011 ; Vol. 300, No. 3.
@article{8a7f9872abf94cb9be73f5319f91ccd9,
title = "Endothelial ischemia-reperfusion injury in humans: Association with age and habitual exercise",
abstract = "Advancing age is a major risk factor for coronary artery disease. Endothelial dysfunction accompanied by increased oxidative stress and inflammation with aging may predispose older arteries to greater ischemia-reperfusion (I/R) injury. Because coronary artery ischemia cannot be induced safely, the effects of age and habitual endurance exercise on endothelial I/R injury have not been determined in humans. Using the brachial artery as a surrogate model of the coronary arteries, endothelial function, assessed by brachial artery flow-mediated dilation (FMD), was measured before and after 20 min of continuous forearm occlusion in young sedentary (n = 10, 24 ± 2 yr) and middle-aged (n = 9, 48 ± 2 yr) sedentary adults to gain insight into the effects of primary aging on endothelial I/R injury. Young (n = 9, 25 ± 1 yr) and middle-aged endurance-trained (n = 9, 50 ± 2 yr) adults were also studied to determine whether habitual exercise provides protection from I/R injury. Fifteen minutes after ischemic injury, FMD decreased significantly by 37{\%} in young sedentary, 35{\%} in young endurancetrained, 68{\%} in middle-aged sedentary, and 50{\%} in middle-aged endurance-trained subjects. FMD returned to baseline levels within 30 min in young sedentary and endurance-trained subjects but remained depressed in middle-aged sedentary and endurance-trained subjects. Circulating markers of antioxidant capacity and inflammation were not related to FMD. In conclusion, advancing age is associated with a greater magnitude and delayed recovery from endothelial I/R injury in humans. Habitual endurance exercise may provide partial protection to the endothelium against this form of I/R injury with advancing age.",
keywords = "Brachial artery, Endothelium, Flow-mediated dilation",
author = "DeVan, {Allison E.} and Daniel Umpierre and Harrison, {Michelle L.} and Lin, {Hsin Fu} and Takashi Tarumi and Renzi, {Christopher P.} and Mandeep Dhindsa and Hunter, {Stacy D.} and Hirofumi Tanaka",
year = "2011",
month = "3",
day = "1",
doi = "10.1152/ajpheart.00845.2010",
language = "English (US)",
volume = "300",
journal = "American Journal of Physiology - Heart and Circulatory Physiology",
issn = "0363-6135",
publisher = "American Physiological Society",
number = "3",

}

TY - JOUR

T1 - Endothelial ischemia-reperfusion injury in humans

T2 - Association with age and habitual exercise

AU - DeVan, Allison E.

AU - Umpierre, Daniel

AU - Harrison, Michelle L.

AU - Lin, Hsin Fu

AU - Tarumi, Takashi

AU - Renzi, Christopher P.

AU - Dhindsa, Mandeep

AU - Hunter, Stacy D.

AU - Tanaka, Hirofumi

PY - 2011/3/1

Y1 - 2011/3/1

N2 - Advancing age is a major risk factor for coronary artery disease. Endothelial dysfunction accompanied by increased oxidative stress and inflammation with aging may predispose older arteries to greater ischemia-reperfusion (I/R) injury. Because coronary artery ischemia cannot be induced safely, the effects of age and habitual endurance exercise on endothelial I/R injury have not been determined in humans. Using the brachial artery as a surrogate model of the coronary arteries, endothelial function, assessed by brachial artery flow-mediated dilation (FMD), was measured before and after 20 min of continuous forearm occlusion in young sedentary (n = 10, 24 ± 2 yr) and middle-aged (n = 9, 48 ± 2 yr) sedentary adults to gain insight into the effects of primary aging on endothelial I/R injury. Young (n = 9, 25 ± 1 yr) and middle-aged endurance-trained (n = 9, 50 ± 2 yr) adults were also studied to determine whether habitual exercise provides protection from I/R injury. Fifteen minutes after ischemic injury, FMD decreased significantly by 37% in young sedentary, 35% in young endurancetrained, 68% in middle-aged sedentary, and 50% in middle-aged endurance-trained subjects. FMD returned to baseline levels within 30 min in young sedentary and endurance-trained subjects but remained depressed in middle-aged sedentary and endurance-trained subjects. Circulating markers of antioxidant capacity and inflammation were not related to FMD. In conclusion, advancing age is associated with a greater magnitude and delayed recovery from endothelial I/R injury in humans. Habitual endurance exercise may provide partial protection to the endothelium against this form of I/R injury with advancing age.

AB - Advancing age is a major risk factor for coronary artery disease. Endothelial dysfunction accompanied by increased oxidative stress and inflammation with aging may predispose older arteries to greater ischemia-reperfusion (I/R) injury. Because coronary artery ischemia cannot be induced safely, the effects of age and habitual endurance exercise on endothelial I/R injury have not been determined in humans. Using the brachial artery as a surrogate model of the coronary arteries, endothelial function, assessed by brachial artery flow-mediated dilation (FMD), was measured before and after 20 min of continuous forearm occlusion in young sedentary (n = 10, 24 ± 2 yr) and middle-aged (n = 9, 48 ± 2 yr) sedentary adults to gain insight into the effects of primary aging on endothelial I/R injury. Young (n = 9, 25 ± 1 yr) and middle-aged endurance-trained (n = 9, 50 ± 2 yr) adults were also studied to determine whether habitual exercise provides protection from I/R injury. Fifteen minutes after ischemic injury, FMD decreased significantly by 37% in young sedentary, 35% in young endurancetrained, 68% in middle-aged sedentary, and 50% in middle-aged endurance-trained subjects. FMD returned to baseline levels within 30 min in young sedentary and endurance-trained subjects but remained depressed in middle-aged sedentary and endurance-trained subjects. Circulating markers of antioxidant capacity and inflammation were not related to FMD. In conclusion, advancing age is associated with a greater magnitude and delayed recovery from endothelial I/R injury in humans. Habitual endurance exercise may provide partial protection to the endothelium against this form of I/R injury with advancing age.

KW - Brachial artery

KW - Endothelium

KW - Flow-mediated dilation

UR - http://www.scopus.com/inward/record.url?scp=79955051078&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=79955051078&partnerID=8YFLogxK

U2 - 10.1152/ajpheart.00845.2010

DO - 10.1152/ajpheart.00845.2010

M3 - Article

C2 - 21239631

AN - SCOPUS:79955051078

VL - 300

JO - American Journal of Physiology - Heart and Circulatory Physiology

JF - American Journal of Physiology - Heart and Circulatory Physiology

SN - 0363-6135

IS - 3

ER -