Endothelial-monocyte activating polypeptide II alters fibronectin based endothelial cell adhesion and matrix assembly via alpha5 beta1 integrin

Margaret A. Schwarz, Hiahua Zheng, Jie Liu, Siobhan Corbett, Roderich E. Schwarz

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

Mature Endothelial-Monocyte Activating Polypeptide (mEMAP) II functions as a potent antiangiogenic peptide. Although the anti-tumor effect of mEMAP II has been described, little is known regarding its mechanism of action. Observations that mEMAP II induced apoptosis only in a subset of migrating and proliferating endothelial cells (EC) suggests a targeted effect on cells engaged in angiogenic activities which are known to rely upon cell adhesion and migration. Indeed, we demonstrate that mEMAP II inhibited fibronectin (FN) dependent microvascular EC (MEC) adhesion and spreading and we show that this depends upon the alpha5 beta1 integrin. Immunofluorescence analysis demonstrated that mEMAP II-dependent blockade of FN-alpha5 beta1 interactions was associated with disassembly of both actin stress fiber networks and FN matrix. These findings suggest that mEMAP II blocks MEC adhesion and spreading on fibronectin, via a direct interaction with the integrin alpha5 beta1, thus implicating that alpha5 integrin may be a mediator of mEMAP II's antiangiogenic function.

Original languageEnglish (US)
Pages (from-to)229-239
Number of pages11
JournalExperimental Cell Research
Volume311
Issue number2
DOIs
StatePublished - Dec 10 2005

Fingerprint

CD29 Antigens
Fibronectins
Cell Adhesion
Endothelial Cells
Integrin alpha5
Stress Fibers
small inducible cytokine subfamily E, member 1
Cell Movement
Fluorescent Antibody Technique
Actins
Apoptosis
Peptides

Keywords

  • Adhesion
  • alphaV beta3
  • Anti-angiogenesis
  • EMAP II
  • Integrin
  • Microvascular endothelium
  • P43

ASJC Scopus subject areas

  • Cell Biology

Cite this

Endothelial-monocyte activating polypeptide II alters fibronectin based endothelial cell adhesion and matrix assembly via alpha5 beta1 integrin. / Schwarz, Margaret A.; Zheng, Hiahua; Liu, Jie; Corbett, Siobhan; Schwarz, Roderich E.

In: Experimental Cell Research, Vol. 311, No. 2, 10.12.2005, p. 229-239.

Research output: Contribution to journalArticle

Schwarz, Margaret A. ; Zheng, Hiahua ; Liu, Jie ; Corbett, Siobhan ; Schwarz, Roderich E. / Endothelial-monocyte activating polypeptide II alters fibronectin based endothelial cell adhesion and matrix assembly via alpha5 beta1 integrin. In: Experimental Cell Research. 2005 ; Vol. 311, No. 2. pp. 229-239.
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