Endothelin-1 and its receptors in human testis

M. Maggi, T. Barni, C. Orlando, G. Fantoni, G. Finetti, G. B. Vannelli, R. Mancina, L. Gloria, L. Bonaccorsi, Masashi Yanagisawa, G. Forti

Research output: Contribution to journalArticle

41 Citations (Scopus)

Abstract

We have previously found the presence of endothelin (ET) recaptor and ET- like immunoreactivity in rat testis. We now extend our studies from rat to human testis. We found expression of a specific transcript for ET-1 and ET- 1-like immunoreactivity in human testis. Positive staining was confined to the Sertoli calls of the tubular compartment, although few peritubular and interstitial cells were also stained. We also identified specific ET(A) and ET(B) receptor transcripts in human testis; ET(A) expression was more abundant than the ET(B) expression. Mathematical analysis of multiple self- and cress-competition studies among [125I]ET-1, [125I]ET-3, and analogues confirmed the presence of the ET(A) and ET(B) isoreceptors. In testicular homogenates, the ET(A) receptor was sevenfold more concentrated than the ET(B) receptor. In order to localize the receptors, we performed [125I]ET-1 autoradiography. Binding sites were mostly concentrated into the seminiferous tubules, although interstitial and peritubular myoid cells were also positive. Within the seminiferous tubules, [125I]ET-1 binding sites were confined to primary and secondary spermatocytes and early spermatids, whereas Sertoli cells were negative. We were unable to demonstrate the presence of functional ET receptors in ejaculated spermatozoa. Because ET-like immunoreactivity was present in Sertoli cells, we next asked whether authentic ET-1 is present in human seminal fluid and represents a good index for Sertoli cell function. Reverse-phase high- performance liquid chromatography analysis of ET-like immunoreactivity in seminal fluid indicated that most of the detected peptides correspond to the ET-1 precursor, big-ET-1. The seminal concentration of ET-like immunoreactivity was similar in normospermic, oligospermic, azoospermic, and vasectomized men, indicating that ETS are produced in different parts of the male genital tract and that they do not represent an useful tool for the diagnosis of male reproductive diseases. In conclusion, this study demonstrated, for the first time, the presence of ET-1 and its receptors in human testis.

Original languageEnglish (US)
Pages (from-to)213-224
Number of pages12
JournalJournal of Andrology
Volume16
Issue number3
StatePublished - 1995

Fingerprint

Endothelin A Receptors
Endothelins
Testis
Endothelin-1
Sertoli Cells
Endothelin B Receptors
Seminiferous Tubules
Binding Sites
Endothelin-3
Brassicaceae
Endothelin Receptors
Spermatocytes
Spermatids
Reverse-Phase Chromatography
Autoradiography
Spermatozoa
High Pressure Liquid Chromatography
Staining and Labeling

Keywords

  • male infertility
  • paracrine/autocrine factors
  • Seminal fluid

ASJC Scopus subject areas

  • Endocrinology

Cite this

Maggi, M., Barni, T., Orlando, C., Fantoni, G., Finetti, G., Vannelli, G. B., ... Forti, G. (1995). Endothelin-1 and its receptors in human testis. Journal of Andrology, 16(3), 213-224.

Endothelin-1 and its receptors in human testis. / Maggi, M.; Barni, T.; Orlando, C.; Fantoni, G.; Finetti, G.; Vannelli, G. B.; Mancina, R.; Gloria, L.; Bonaccorsi, L.; Yanagisawa, Masashi; Forti, G.

In: Journal of Andrology, Vol. 16, No. 3, 1995, p. 213-224.

Research output: Contribution to journalArticle

Maggi, M, Barni, T, Orlando, C, Fantoni, G, Finetti, G, Vannelli, GB, Mancina, R, Gloria, L, Bonaccorsi, L, Yanagisawa, M & Forti, G 1995, 'Endothelin-1 and its receptors in human testis', Journal of Andrology, vol. 16, no. 3, pp. 213-224.
Maggi M, Barni T, Orlando C, Fantoni G, Finetti G, Vannelli GB et al. Endothelin-1 and its receptors in human testis. Journal of Andrology. 1995;16(3):213-224.
Maggi, M. ; Barni, T. ; Orlando, C. ; Fantoni, G. ; Finetti, G. ; Vannelli, G. B. ; Mancina, R. ; Gloria, L. ; Bonaccorsi, L. ; Yanagisawa, Masashi ; Forti, G. / Endothelin-1 and its receptors in human testis. In: Journal of Andrology. 1995 ; Vol. 16, No. 3. pp. 213-224.
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AU - Maggi, M.

AU - Barni, T.

AU - Orlando, C.

AU - Fantoni, G.

AU - Finetti, G.

AU - Vannelli, G. B.

AU - Mancina, R.

AU - Gloria, L.

AU - Bonaccorsi, L.

AU - Yanagisawa, Masashi

AU - Forti, G.

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N2 - We have previously found the presence of endothelin (ET) recaptor and ET- like immunoreactivity in rat testis. We now extend our studies from rat to human testis. We found expression of a specific transcript for ET-1 and ET- 1-like immunoreactivity in human testis. Positive staining was confined to the Sertoli calls of the tubular compartment, although few peritubular and interstitial cells were also stained. We also identified specific ET(A) and ET(B) receptor transcripts in human testis; ET(A) expression was more abundant than the ET(B) expression. Mathematical analysis of multiple self- and cress-competition studies among [125I]ET-1, [125I]ET-3, and analogues confirmed the presence of the ET(A) and ET(B) isoreceptors. In testicular homogenates, the ET(A) receptor was sevenfold more concentrated than the ET(B) receptor. In order to localize the receptors, we performed [125I]ET-1 autoradiography. Binding sites were mostly concentrated into the seminiferous tubules, although interstitial and peritubular myoid cells were also positive. Within the seminiferous tubules, [125I]ET-1 binding sites were confined to primary and secondary spermatocytes and early spermatids, whereas Sertoli cells were negative. We were unable to demonstrate the presence of functional ET receptors in ejaculated spermatozoa. Because ET-like immunoreactivity was present in Sertoli cells, we next asked whether authentic ET-1 is present in human seminal fluid and represents a good index for Sertoli cell function. Reverse-phase high- performance liquid chromatography analysis of ET-like immunoreactivity in seminal fluid indicated that most of the detected peptides correspond to the ET-1 precursor, big-ET-1. The seminal concentration of ET-like immunoreactivity was similar in normospermic, oligospermic, azoospermic, and vasectomized men, indicating that ETS are produced in different parts of the male genital tract and that they do not represent an useful tool for the diagnosis of male reproductive diseases. In conclusion, this study demonstrated, for the first time, the presence of ET-1 and its receptors in human testis.

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