Endothelin-1 in luteal tissue

S. Usuki, N. Suzuki, H. Matsumoto, M. Yanagisawa, T. Masaki

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

The aim of this study was to immunologically and biologically detect endothelin-1 (ET-1) in rat corpora lutea (CL). Recently, we established a highly sensitive and specific sandwich-enzyme immunoassay (EIA) for ET-1. Using this assay, the presence of ET-1 was investigated in superovulated ovaries, induced with pregnant mare's serum gonadotropin (PMSG) and human chorionic gonadotropin (hCG), and ovaries from pseudopregnant rats, induced by cervical stimulation. A high concentration of immunoreactive endothelin-1 (ir-ET-1) was found in the CL. On reverse phase-high performance liquid chromatography (RP-HPLC) coupled with EIA, ir-ET-1 was exclusively eluted at the same position as synthetic ET-1, indicating that ir-ET-1 is identical to ET-1. The level of ir-ET-1 was significantly (P < 0.001) higher in the CL 7 days after hCG injection than it was 4 days after hCG injection. On day 7 of pseudopregnancy (PSP), the ir-ET-1 level was also significantly (P < 0.001) higher than on day 4 of PSP. These results demonstrated that ET-1 is present in a high concentration in the CL, suggesting a new intraovarian peptide which may have a physiological function in the ovary and which may vary in quantity according to the age of the CL.

Original languageEnglish (US)
Pages (from-to)147-151
Number of pages5
JournalMolecular and Cellular Endocrinology
Volume80
Issue number1-3
DOIs
StatePublished - 1991

Fingerprint

Corpus Luteum
Endothelin-1
Tissue
Chorionic Gonadotropin
Pseudopregnancy
Ovary
Immunoenzyme Techniques
Rats
Equine Gonadotropins
Injections
High performance liquid chromatography
Enzymes
Assays
High Pressure Liquid Chromatography

Keywords

  • Corpus luteum
  • Endothelin-1

ASJC Scopus subject areas

  • Endocrinology
  • Endocrinology, Diabetes and Metabolism

Cite this

Endothelin-1 in luteal tissue. / Usuki, S.; Suzuki, N.; Matsumoto, H.; Yanagisawa, M.; Masaki, T.

In: Molecular and Cellular Endocrinology, Vol. 80, No. 1-3, 1991, p. 147-151.

Research output: Contribution to journalArticle

Usuki, S, Suzuki, N, Matsumoto, H, Yanagisawa, M & Masaki, T 1991, 'Endothelin-1 in luteal tissue', Molecular and Cellular Endocrinology, vol. 80, no. 1-3, pp. 147-151. https://doi.org/10.1016/0303-7207(91)90151-H
Usuki, S. ; Suzuki, N. ; Matsumoto, H. ; Yanagisawa, M. ; Masaki, T. / Endothelin-1 in luteal tissue. In: Molecular and Cellular Endocrinology. 1991 ; Vol. 80, No. 1-3. pp. 147-151.
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