TY - JOUR
T1 - Endothelin-3 is a novel neuropeptide
T2 - Isolation and sequence determination of endothelin-1 and endothelin-3 in porcine brain
AU - Shinmi, Osamu
AU - Kimura, Sadao
AU - Sawamura, Tatsuya
AU - Sugita, Yoshiki
AU - Yoshizawa, Toshihiro
AU - Uchiyama, Yasuo
AU - Yanagisawa, Masashi
AU - Goto, Katsutoshi
AU - Masaki, Tomoh
AU - Kanazawa, Ichiro
PY - 1989/10/16
Y1 - 1989/10/16
N2 - The molecular forms of endothelin (ET) related peptides were investigated in porcine brain by using high performance liquid chromatography coupled with three specific radioimmunoassays. ET-1 and its oxidized form were isolated and sequenced as in the case of porcine spinal cord. A very small amount of big ET-1 (1-39) and its C-terminal fragment (big ET-1(22-39)) were also detected. Furthermore, immunoreactive (ir)-ET-3 was isolated and sequenced; its partial primary structure was identical to that of human (rat) ET-3. The concentrations of ir-ET-1 and ir-ET-3 in porcine brain were 140 fmol/g tissue and 5 fmol/g tissue, respectively. These results indicate that besides ET-1, ET-3 is a novel neuropeptide in the central nervous system.
AB - The molecular forms of endothelin (ET) related peptides were investigated in porcine brain by using high performance liquid chromatography coupled with three specific radioimmunoassays. ET-1 and its oxidized form were isolated and sequenced as in the case of porcine spinal cord. A very small amount of big ET-1 (1-39) and its C-terminal fragment (big ET-1(22-39)) were also detected. Furthermore, immunoreactive (ir)-ET-3 was isolated and sequenced; its partial primary structure was identical to that of human (rat) ET-3. The concentrations of ir-ET-1 and ir-ET-3 in porcine brain were 140 fmol/g tissue and 5 fmol/g tissue, respectively. These results indicate that besides ET-1, ET-3 is a novel neuropeptide in the central nervous system.
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U2 - 10.1016/0006-291X(89)91760-9
DO - 10.1016/0006-291X(89)91760-9
M3 - Article
C2 - 2508640
AN - SCOPUS:0024421897
SN - 0006-291X
VL - 164
SP - 587
EP - 593
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 1
ER -