Endothelin A receptor is necessary for O2 constriction but not closure of ductus arteriosus

F. Coceani, Y. A. Liu, E. Seidlitz, L. Kelsey, T. Kuwaki, C. Ackerley, M. Yanagisawa

Research output: Contribution to journalArticle

55 Scopus citations

Abstract

In vitro and in vivo techniques were developed with genetically modified mice to determine whether endothelin-1 (ET-1) functions as an O2 mediator in closure of the ductus arteriosus (DA) at birth. Wild-type CD-1 and 129/SvEv mice with ET(A) receptor -/-, +/-, and +/+ genotypes were used. Isolated DA from term ET(A) +/+ fetuses contracted to O2 (5-95%) and a thromboxane A2 analog (ONO-11113, 0.1 μM). Instead, ET-1 elicited a dual response with weak relaxation (0.1 nM) preceding contraction (1-100 nM). Indomethacin (2.8 μM) was also a constrictor. ET(A) -/DA, unlike ET(A) +/+ DA, contracted marginally to O2 and ET-1 but responded to ONO-11113. O2 contraction was also reduced in ET(A) +/- DA. In vivo, DA constricted equally in tracheotomized ET(A) -/- and ET(A) +/+ newborns. Conversely, no DA constriction was seen in hyperoxic ET(A) -/- fetuses in utero, although it occurred in ET(A) +/+ and +/- littermates. We conclude that ET-1 mediates the DA constrictor response to O2. Without ET-1, however, the vessel still closes postnatally, conceivably caused by the withdrawal of relaxing influence(s).

Original languageEnglish (US)
Pages (from-to)H1521-H1531
JournalAmerican Journal of Physiology - Heart and Circulatory Physiology
Volume277
Issue number4 46-4
DOIs
StatePublished - Oct 1999

Keywords

  • Endothelin
  • Oxygen

ASJC Scopus subject areas

  • Physiology
  • Cardiology and Cardiovascular Medicine
  • Physiology (medical)

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