Endothelin-B receptors activate Gai3

Kenichiro Kitamura, Naoki Shiraishi, William D. Singer, Mary E. Handlogten, Kimio Tomita, R. Tyler Miller

Research output: Contribution to journalArticle

Abstract

Endo-thelin (ET) receptors activate heterotrimeric G proteins that are members of the Gi; Gq, and Gs families but may also activate members of other families such as 60112/13. Gotis has multiple complex cellular effects that are similar to those of ET. We studied the ability of ET receptors to activate Gais using an assay for G protein a-chain activation that is based on the fact that an activated (GTP-bound) a-chain is resistant to trypsinization compared with an inactive (GDP-bound) a-chain. Nonhydrolyzable guanine nucleotides and AlMgF protected Ga13 from degradation by trypsin. In membranes from human embryonic kidney 293 cells that coexpress ETβ receptors and ai3, ET-3 and 5'-guanylylimidodiphosphate [Gpp(NH)p] increased the protection of 013 compared with Gpp(NH)p alone. The specificity of ETβ receptor-aja coupling was documented by showing that β2 receptors and isoproterenol or ETA receptors and ET-1 did not activate aïs and that a specific antagonist for ETβ receptors blocked ET-3-dependent activation of ai3. Copyright a> 1999 the American Physiological Society.

Original languageEnglish (US)
JournalAmerican Journal of Physiology
Volume276
Issue number4 PART 1
StatePublished - 1999

Fingerprint

Guanylyl Imidodiphosphate
Endothelin B Receptors
Heterotrimeric GTP-Binding Proteins
Aptitude
Guanine Nucleotides
Guanosine Triphosphate
Isoproterenol
GTP-Binding Proteins
Trypsin
Kidney
Membranes

Keywords

  • Cell signaling
  • G protein
  • G protein-coupled receptor

ASJC Scopus subject areas

  • Physiology (medical)

Cite this

Kitamura, K., Shiraishi, N., Singer, W. D., Handlogten, M. E., Tomita, K., & Tyler Miller, R. (1999). Endothelin-B receptors activate Gai3. American Journal of Physiology, 276(4 PART 1).

Endothelin-B receptors activate Gai3. / Kitamura, Kenichiro; Shiraishi, Naoki; Singer, William D.; Handlogten, Mary E.; Tomita, Kimio; Tyler Miller, R.

In: American Journal of Physiology, Vol. 276, No. 4 PART 1, 1999.

Research output: Contribution to journalArticle

Kitamura, K, Shiraishi, N, Singer, WD, Handlogten, ME, Tomita, K & Tyler Miller, R 1999, 'Endothelin-B receptors activate Gai3', American Journal of Physiology, vol. 276, no. 4 PART 1.
Kitamura K, Shiraishi N, Singer WD, Handlogten ME, Tomita K, Tyler Miller R. Endothelin-B receptors activate Gai3. American Journal of Physiology. 1999;276(4 PART 1).
Kitamura, Kenichiro ; Shiraishi, Naoki ; Singer, William D. ; Handlogten, Mary E. ; Tomita, Kimio ; Tyler Miller, R. / Endothelin-B receptors activate Gai3. In: American Journal of Physiology. 1999 ; Vol. 276, No. 4 PART 1.
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