Endothelin is a potent secretagogue for atrial natriuretic peptide in cultured rat atrial myocytes

Yuka Fukuda, Yukio Hirata, Hiroki Yoshimi, Takatsugu Kojima, Yohnosuke Kobayashi, Masashi Yanagisawa, Tomoh Masaki

Research output: Contribution to journalArticle

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Abstract

Using cultured neonatal rat atrial cardiocytes, we have studied the effect of synthetic porcine endothelin (pET), a novel potent vasoconstrictor isolated from endothelial cells, on the release of immunoreactive (IR) rat atrial natriuretic peptide (rANP). pET stimulated IR-rANP secretion in a dose-dependent manner (10-10-10-7M) with an approximate half-maximally stimulatory dose of 2×10-10M. The pET-induced IR-rANP secretion was attenuated by Ca2+-channel blocker nicardipine, but no further stimulation was induced when combined with a Ca2+-channel agonist BAY-K 8644. pET in combination with tetradecanoyl-phorbol-acetate resulted in a synergistic effect on IR-rANP secretion. These data suggest that ET may play as an endogenous secretagogue for rANP by modulating Ca2+ influx through the voltage-dependent Ca2+-channels in atrial cardiocytes.

Original languageEnglish (US)
Pages (from-to)167-172
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume155
Issue number1
DOIs
StatePublished - Aug 30 1988

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Endothelins
Atrial Natriuretic Factor
Muscle Cells
Rats
Swine
Nicardipine
Endothelial cells
Vasoconstrictor Agents
Acetates
Endothelial Cells
rat atrial natriuretic peptide
Electric potential

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Endothelin is a potent secretagogue for atrial natriuretic peptide in cultured rat atrial myocytes. / Fukuda, Yuka; Hirata, Yukio; Yoshimi, Hiroki; Kojima, Takatsugu; Kobayashi, Yohnosuke; Yanagisawa, Masashi; Masaki, Tomoh.

In: Biochemical and Biophysical Research Communications, Vol. 155, No. 1, 30.08.1988, p. 167-172.

Research output: Contribution to journalArticle

Fukuda, Yuka ; Hirata, Yukio ; Yoshimi, Hiroki ; Kojima, Takatsugu ; Kobayashi, Yohnosuke ; Yanagisawa, Masashi ; Masaki, Tomoh. / Endothelin is a potent secretagogue for atrial natriuretic peptide in cultured rat atrial myocytes. In: Biochemical and Biophysical Research Communications. 1988 ; Vol. 155, No. 1. pp. 167-172.
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