Endothelin receptors in human parathyroid gland

Satoru Eguchi, Yukio Hirata, Taihei Imai, Kazuo Kanno, Takashi Akiba, Aiji Sakamoto, Masashi Yanagisawa, Tomoh Masaki, Fumiaki Marumo

Research output: Contribution to journalArticlepeer-review

39 Scopus citations

Abstract

We studied whether specific receptors for endothelins (ETs) exist in human parathyroid tissues and whether ETs may have any effect on secretion of PTH from parathyroid cells. Binding studies using [125I]ET-1 to the parathyroid membranes obtained from patients with hyperparathyroidism (2 adenomas, 2 hyperplasias) revealed that ET-1 competitively inhibited the binding of [125I]ET-1 to the membranes (the apparent Kd: 62±18 pM), whereas ET-3 showed biphasic and less steep inhibition curve than ET-1 in all tissue membranes examined. Northern blot analysis of poly(A)+ RNA from the parathyroid adenoma clearly demonstrated gene expression of both ETA and ETB receptors as well as preproET-1. ET-1 inhibited basal PTH secretion from dispersed adenoma cells more potently than ET-3. The present study clearly demonstrates the presence of both ETA and ETB receptor subtypes in human parathyroid tissues through which ETs may modulate PTH secretion in an autocrine and/or paracrine manner.

Original languageEnglish (US)
Pages (from-to)1448-1455
Number of pages8
JournalBiochemical and Biophysical Research Communications
Volume184
Issue number3
DOIs
StatePublished - May 15 1992

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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