Endotoxin and pro-inflammatory cytokines stimulate endothelin-1 expression and release by airway epithelial cells

J. Nakano, H. Takizawa, T. Ohtoshi, S. Shoji, M. Yamaguchi, A. Ishii, Masashi Yanagisawa, K. Ito

Research output: Contribution to journalArticle

80 Citations (Scopus)

Abstract

Endothelin is a potent bronchoconstrictor peptide first identified as a novel vasoconstrictor produced by vascular endothelial cells. Recent reports suggest that airway epithelial cells are also capable of releasing this active peptide. To investigate the regulatory mechanism of endothelin expression, we studied the effects of endotoxin and pro-inflammatory cytokines such as interleukin-1 and tumour necrosis factor on the expression and release of endothelin-1 by airway epithelial cells. Both endotoxin and the cytokines stimulated endothelin-1 release by human bronchial epithelial cells. Northern blot analysis showed increased expression of preproendothelin-1 mRNA by these factors. These results suggested that airway epithelial cells might play a role in the local airway smooth muscle tone through the production of endothelin, which might be upregulated by inflammatory products in the airways.

Original languageEnglish (US)
Pages (from-to)330-336
Number of pages7
JournalClinical and Experimental Allergy
Volume24
Issue number4
DOIs
StatePublished - 1994

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Endothelin-1
Endotoxins
Endothelins
Epithelial Cells
Cytokines
Bronchoconstrictor Agents
Peptides
Vasoconstrictor Agents
Interleukin-1
Northern Blotting
Smooth Muscle
Endothelial Cells
Tumor Necrosis Factor-alpha
Messenger RNA

ASJC Scopus subject areas

  • Immunology

Cite this

Endotoxin and pro-inflammatory cytokines stimulate endothelin-1 expression and release by airway epithelial cells. / Nakano, J.; Takizawa, H.; Ohtoshi, T.; Shoji, S.; Yamaguchi, M.; Ishii, A.; Yanagisawa, Masashi; Ito, K.

In: Clinical and Experimental Allergy, Vol. 24, No. 4, 1994, p. 330-336.

Research output: Contribution to journalArticle

Nakano, J. ; Takizawa, H. ; Ohtoshi, T. ; Shoji, S. ; Yamaguchi, M. ; Ishii, A. ; Yanagisawa, Masashi ; Ito, K. / Endotoxin and pro-inflammatory cytokines stimulate endothelin-1 expression and release by airway epithelial cells. In: Clinical and Experimental Allergy. 1994 ; Vol. 24, No. 4. pp. 330-336.
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