Endotoxin stimulation of liver parenchymal cell phosphofructokinase activity requires nonparenchymal cells

B. C. Miller, K. Uyeda, G. L. Cottam

Research output: Contribution to journalArticle

2 Scopus citations

Abstract

The rate of carbohydrate flux through phosphofructokinase (measured as the rate of [3‐3H]glucose detritiation) was increased fourfold in rat liver parenchymal cells incubated with conditioned medium from lipopolysaccharide‐stimulated adherent liver non‐parenchymal cells. The rate was not affected in parenchymal cells incubated either with lipopolysaccharide directly or with conditioned medium from non‐stimulated non‐parenchymal cells. The stimulation of carbohydrate flux through phosphofructokinase by conditioned medium was not duplicated by peptide cytokines known to be released by lipopolysaccharide‐activated liver non‐parenchymal cells (interleukin‐1, interleukin‐6, tumor necrosis factor‐α, and transforming growth factor‐β) or platelet activating factor. Furthermore, formation of the active conditioned medium was not prevented by inclusion of cycloheximide or dexamethasone to inhibit cytokine synthesis, or indomethacin or BW755c to inhibit arachidonic acid metabolism, during lipopolysaccharide‐stimulation of the non‐parenchymal cells. The results indicate that intercellular communication between lipopolysaccharide‐stimulated liver non‐parenchymal cells and parenchymal cells by soluble mediators is responsible for the stimulation of liver phosphofructokinase activity during endotoxin‐induced shock. Studies to isolate and identify the factor(s) in the conditioned medium are currently in progress.

Original languageEnglish (US)
Pages (from-to)593-598
Number of pages6
JournalEuropean Journal of Biochemistry
Volume203
Issue number3
DOIs
StatePublished - Feb 1992

ASJC Scopus subject areas

  • Biochemistry

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