Endotypes of difficult-to-control asthma in inner-city African American children

K. R. Brown, R. Z. Krouse, A. Calatroni, C. M. Visness, U. Sivaprasad, C. M. Kercsmar, E. C. Matsui, J. B. West, M. M. Makhija, M. A. Gill, H. Kim, M. Kattan, D. Pillai, J. E. Gern, W. W. Busse, A. Togias, A. H. Liu, G. K.Khurana Hershey

Research output: Contribution to journalArticle

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Abstract

African Americans have higher rates of asthma prevalence, morbidity, and mortality in comparison with other racial groups. We sought to characterize endotypes of childhood asthma severity in African American patients in an inner-city pediatric asthma population. Baseline blood neutrophils, blood eosinophils, and 38 serum cytokine levels were measured in a sample of 235 asthmatic children (6–17 years) enrolled in the NIAID (National Institute of Allergy and Infectious Diseases)-sponsored Asthma Phenotypes in the Inner City (APIC) study (ICAC (Inner City Asthma Consortium)-19). Cytokines were quantified using a MILLIPLEX panel and analyzed on a Luminex analyzer. Patients were classified as Easy-to-Control or Difficult-to-Control based on the required dose of controller medications over one year of prospective management. A multivariate variable selection procedure was used to select cytokines associated with Difficult-to-Control versus Easy-to-Control asthma, adjusting for age, sex, blood eosinophils, and blood neutrophils. In inner-city African American children, 12 cytokines were significant predictors of Difficult-to-Control asthma (n = 235). CXCL-1, IL-5, IL-8, and IL-17A were positively associated with Difficult-to-Control asthma, while IL-4 and IL-13 were positively associated with Easy-to-Control asthma. Using likelihood ratio testing, it was observed that in addition to blood eosinophils and neutrophils, serum cytokines improved the fit of the model. In an inner-city pediatric population, serum cytokines significantly contributed to the definition of Difficult-to-Control asthma endotypes in African American children. Mixed responses characterized by TH2 (IL-5) and TH17-associated cytokines were associated with Difficult-to-Control asthma. Collectively, these data may contribute to risk stratification of Difficult-to-Control asthma in the African American population.

Original languageEnglish (US)
Article numbere0180778
JournalPLoS One
Volume12
Issue number7
DOIs
StatePublished - Jul 1 2017

Fingerprint

asthma
African Americans
Asthma
Cytokines
cytokines
Blood
eosinophils
blood serum
Eosinophils
Pediatrics
blood
Interleukin-5
neutrophils
interleukin-5
Neutrophils
Allergies
National Institute of Allergy and Infectious Diseases (U.S.)
Serum
Interleukin-13
Interleukin-17

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

Brown, K. R., Krouse, R. Z., Calatroni, A., Visness, C. M., Sivaprasad, U., Kercsmar, C. M., ... Hershey, G. K. K. (2017). Endotypes of difficult-to-control asthma in inner-city African American children. PLoS One, 12(7), [e0180778]. https://doi.org/10.1371/journal.pone.0180778

Endotypes of difficult-to-control asthma in inner-city African American children. / Brown, K. R.; Krouse, R. Z.; Calatroni, A.; Visness, C. M.; Sivaprasad, U.; Kercsmar, C. M.; Matsui, E. C.; West, J. B.; Makhija, M. M.; Gill, M. A.; Kim, H.; Kattan, M.; Pillai, D.; Gern, J. E.; Busse, W. W.; Togias, A.; Liu, A. H.; Hershey, G. K.Khurana.

In: PLoS One, Vol. 12, No. 7, e0180778, 01.07.2017.

Research output: Contribution to journalArticle

Brown, KR, Krouse, RZ, Calatroni, A, Visness, CM, Sivaprasad, U, Kercsmar, CM, Matsui, EC, West, JB, Makhija, MM, Gill, MA, Kim, H, Kattan, M, Pillai, D, Gern, JE, Busse, WW, Togias, A, Liu, AH & Hershey, GKK 2017, 'Endotypes of difficult-to-control asthma in inner-city African American children', PLoS One, vol. 12, no. 7, e0180778. https://doi.org/10.1371/journal.pone.0180778
Brown KR, Krouse RZ, Calatroni A, Visness CM, Sivaprasad U, Kercsmar CM et al. Endotypes of difficult-to-control asthma in inner-city African American children. PLoS One. 2017 Jul 1;12(7). e0180778. https://doi.org/10.1371/journal.pone.0180778
Brown, K. R. ; Krouse, R. Z. ; Calatroni, A. ; Visness, C. M. ; Sivaprasad, U. ; Kercsmar, C. M. ; Matsui, E. C. ; West, J. B. ; Makhija, M. M. ; Gill, M. A. ; Kim, H. ; Kattan, M. ; Pillai, D. ; Gern, J. E. ; Busse, W. W. ; Togias, A. ; Liu, A. H. ; Hershey, G. K.Khurana. / Endotypes of difficult-to-control asthma in inner-city African American children. In: PLoS One. 2017 ; Vol. 12, No. 7.
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