Enhanced alveolar growth and remodeling in Guinea pigs raised at high altitude

Connie C W Hsia, Juan J Polo Carbayo, Xiao Yan, Dennis J. Bellotto

Research output: Contribution to journalArticle

39 Citations (Scopus)

Abstract

To examine the effects of chronic high altitude (HA) exposure on lung structure during somatic maturation, we raised male weanling guinea pigs at HA (3800 m) for 1, 3, or 6 months, while their respective male littermates were simultaneously raised at low altitude (LA, 1200 m). Under anaesthesia, airway pressure was measured at different lung volumes. The right lung was fixed at a constant airway pressure for morphometric analysis under light and electron microscopy. In animals raised at HA for 1 month, lung volume, alveolar surface area and alveolar-capillary blood volume (Vc) were elevated above LA control values. Following 3-6 months of HA exposure, increases in lung volume and alveolar surface area persisted while the initial increase in Vc normalized. Additional adaptation occurred, including a higher epithelial cell volume, septal tissue volume and capillary surface area, a lower alveolar duct volume and lower harmonic mean diffusion barrier resulting in higher membrane and lung diffusing capacities. These data demonstrate enhanced alveolar septal growth and progressive acinar remodeling during chronic HA exposure with long-term augmentation of alveolar dimensions as well as functional compensation in lung compliance and diffusive gas transport.

Original languageEnglish (US)
Pages (from-to)105-115
Number of pages11
JournalRespiratory Physiology and Neurobiology
Volume147
Issue number1
DOIs
StatePublished - May 12 2005

Fingerprint

Guinea Pigs
Lung
Growth
Lung Volume Measurements
Lung Compliance
Pressure
Blood Volume
Cell Size
Electron Microscopy
Anesthesia
Gases
Epithelial Cells
Light
Membranes

Keywords

  • Alveolar duct
  • Alveolar surface area
  • Alveolar tissue volume
  • Diffusion barrier
  • Hypoxia
  • Lung diffusing capacity
  • Lung growth
  • Membrane diffusing capacity

ASJC Scopus subject areas

  • Physiology
  • Pulmonary and Respiratory Medicine
  • Neuroscience(all)

Cite this

Enhanced alveolar growth and remodeling in Guinea pigs raised at high altitude. / Hsia, Connie C W; Carbayo, Juan J Polo; Yan, Xiao; Bellotto, Dennis J.

In: Respiratory Physiology and Neurobiology, Vol. 147, No. 1, 12.05.2005, p. 105-115.

Research output: Contribution to journalArticle

@article{e24198519bbd4319988cab5966c595df,
title = "Enhanced alveolar growth and remodeling in Guinea pigs raised at high altitude",
abstract = "To examine the effects of chronic high altitude (HA) exposure on lung structure during somatic maturation, we raised male weanling guinea pigs at HA (3800 m) for 1, 3, or 6 months, while their respective male littermates were simultaneously raised at low altitude (LA, 1200 m). Under anaesthesia, airway pressure was measured at different lung volumes. The right lung was fixed at a constant airway pressure for morphometric analysis under light and electron microscopy. In animals raised at HA for 1 month, lung volume, alveolar surface area and alveolar-capillary blood volume (Vc) were elevated above LA control values. Following 3-6 months of HA exposure, increases in lung volume and alveolar surface area persisted while the initial increase in Vc normalized. Additional adaptation occurred, including a higher epithelial cell volume, septal tissue volume and capillary surface area, a lower alveolar duct volume and lower harmonic mean diffusion barrier resulting in higher membrane and lung diffusing capacities. These data demonstrate enhanced alveolar septal growth and progressive acinar remodeling during chronic HA exposure with long-term augmentation of alveolar dimensions as well as functional compensation in lung compliance and diffusive gas transport.",
keywords = "Alveolar duct, Alveolar surface area, Alveolar tissue volume, Diffusion barrier, Hypoxia, Lung diffusing capacity, Lung growth, Membrane diffusing capacity",
author = "Hsia, {Connie C W} and Carbayo, {Juan J Polo} and Xiao Yan and Bellotto, {Dennis J.}",
year = "2005",
month = "5",
day = "12",
doi = "10.1016/j.resp.2005.02.001",
language = "English (US)",
volume = "147",
pages = "105--115",
journal = "Respiratory Physiology and Neurobiology",
issn = "1569-9048",
publisher = "Elsevier",
number = "1",

}

TY - JOUR

T1 - Enhanced alveolar growth and remodeling in Guinea pigs raised at high altitude

AU - Hsia, Connie C W

AU - Carbayo, Juan J Polo

AU - Yan, Xiao

AU - Bellotto, Dennis J.

PY - 2005/5/12

Y1 - 2005/5/12

N2 - To examine the effects of chronic high altitude (HA) exposure on lung structure during somatic maturation, we raised male weanling guinea pigs at HA (3800 m) for 1, 3, or 6 months, while their respective male littermates were simultaneously raised at low altitude (LA, 1200 m). Under anaesthesia, airway pressure was measured at different lung volumes. The right lung was fixed at a constant airway pressure for morphometric analysis under light and electron microscopy. In animals raised at HA for 1 month, lung volume, alveolar surface area and alveolar-capillary blood volume (Vc) were elevated above LA control values. Following 3-6 months of HA exposure, increases in lung volume and alveolar surface area persisted while the initial increase in Vc normalized. Additional adaptation occurred, including a higher epithelial cell volume, septal tissue volume and capillary surface area, a lower alveolar duct volume and lower harmonic mean diffusion barrier resulting in higher membrane and lung diffusing capacities. These data demonstrate enhanced alveolar septal growth and progressive acinar remodeling during chronic HA exposure with long-term augmentation of alveolar dimensions as well as functional compensation in lung compliance and diffusive gas transport.

AB - To examine the effects of chronic high altitude (HA) exposure on lung structure during somatic maturation, we raised male weanling guinea pigs at HA (3800 m) for 1, 3, or 6 months, while their respective male littermates were simultaneously raised at low altitude (LA, 1200 m). Under anaesthesia, airway pressure was measured at different lung volumes. The right lung was fixed at a constant airway pressure for morphometric analysis under light and electron microscopy. In animals raised at HA for 1 month, lung volume, alveolar surface area and alveolar-capillary blood volume (Vc) were elevated above LA control values. Following 3-6 months of HA exposure, increases in lung volume and alveolar surface area persisted while the initial increase in Vc normalized. Additional adaptation occurred, including a higher epithelial cell volume, septal tissue volume and capillary surface area, a lower alveolar duct volume and lower harmonic mean diffusion barrier resulting in higher membrane and lung diffusing capacities. These data demonstrate enhanced alveolar septal growth and progressive acinar remodeling during chronic HA exposure with long-term augmentation of alveolar dimensions as well as functional compensation in lung compliance and diffusive gas transport.

KW - Alveolar duct

KW - Alveolar surface area

KW - Alveolar tissue volume

KW - Diffusion barrier

KW - Hypoxia

KW - Lung diffusing capacity

KW - Lung growth

KW - Membrane diffusing capacity

UR - http://www.scopus.com/inward/record.url?scp=17444362233&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=17444362233&partnerID=8YFLogxK

U2 - 10.1016/j.resp.2005.02.001

DO - 10.1016/j.resp.2005.02.001

M3 - Article

C2 - 15848128

AN - SCOPUS:17444362233

VL - 147

SP - 105

EP - 115

JO - Respiratory Physiology and Neurobiology

JF - Respiratory Physiology and Neurobiology

SN - 1569-9048

IS - 1

ER -