Enhanced biofilm formation and/or cell viability by polyamines through stimulation of response regulators UvrY and CpxR in the two-component signal transducing systems, and ribosome recycling factor

Akihiko Sakamoto, Yusuke Terui, Taku Yamamoto, Takuma Kasahara, Mizuho Nakamura, Hideyuki Tomitori, Kaneyoshi Yamamoto, Akira Ishihama, Anthony J. Michael, Kazuei Igarashi, Keiko Kashiwagi

Research output: Contribution to journalArticle

29 Scopus citations

Abstract

We have reported that polyamines increase cell viability at the stationary phase of cell growth through translational stimulation of ribosome modulation factor, and SpoT and RpoZ proteins involved in the synthesis and function of ppGpp in Escherichia coli. Since biofilm formation is also involved in cell viability, we looked for proteins involved in biofilm formation and cell viability whose synthesis is stimulated by polyamines at the level of translation. It was found that the synthesis of response regulators UvrY and CpxR in the two-component signal transducing systems and ribosome recycling factor (RRF) was increased by polyamines at the level of translation. Polyamine stimulation of the synthesis of UvrY and RRF was dependent on the existence of the inefficient initiation codons UUG and GUG in uvrY and frr mRNA, respectively; and polyamine stimulation of CpxR synthesis was dependent on the existence of an unusual location of a Shine-Dalgarno (SD) sequence in cpxR mRNA. Biofilm formation and cell viability in the absence of polyamines was increased by transformation of modified uvrY and cpxR genes, and cell viability by modified frr gene whose translation occurs effectively without polyamines. The results indicate that polyamines are necessary for both biofilm formation and cell viability.

Original languageEnglish (US)
Pages (from-to)1877-1886
Number of pages10
JournalInternational Journal of Biochemistry and Cell Biology
Volume44
Issue number11
DOIs
StatePublished - Nov 1 2012

Keywords

  • Biofilm formation
  • Cell viability
  • Polyamine modulon
  • Ribosome recycling factor
  • Two-component signal transducing system

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology

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