TY - JOUR
T1 - Enhanced egg-induced immunopathology correlates with high IFN-γ in murine schistosomiasis
T2 - Identification of two epistatic genetic intervals
AU - Rutitzky, Laura I.
AU - Hernandez, Hector J.
AU - Yim, Young Sun
AU - Ricklan, David E.
AU - Finger, Eduardo
AU - Mohan, Chandra
AU - Peter, Inga
AU - Wakeland, Edward K.
AU - Stadecker, Miguel J.
PY - 2005/1/1
Y1 - 2005/1/1
N2 - The genetic basis of dissimilar immunopathology development among mouse strains infected with Schistosoma mansoni is not known. We performed a multipoint parametric linkage analysis on a cohort of F2 mice, offspring of brother-sister mating between (high pathology CBA x low pathology BL/6)F1 mice, to examine whether the observed differences in the type of immune response or the extent of hepatic immunopathology are linked to any particular genomic intervals. The F2 mice exhibited cytokine responses and immunopathologies that revealed a statistically significant correlation between prominent egg Ag-stimulated IFN-γ production by mesenteric lymph node cells and hepatic egg granuloma size. Increased IFN-γ production showed suggestive linkage to a dominant CBA locus on chromosome 1 and a recessive CBA locus on chromosome 5; significantly, there was an epistatic interaction between the two IFN-γ loci. An additional locus with suggestive linkage to granuloma formation and a CBA-recessive mode of inheritance was mapped to centromeric chromosome 13. Our analysis identified the first three genetic regions that appear to influence the immunopathology in murine schistosomiasis; however, further congenic dissection studies will furnish a more precise understanding of the genetic control of this disease.
AB - The genetic basis of dissimilar immunopathology development among mouse strains infected with Schistosoma mansoni is not known. We performed a multipoint parametric linkage analysis on a cohort of F2 mice, offspring of brother-sister mating between (high pathology CBA x low pathology BL/6)F1 mice, to examine whether the observed differences in the type of immune response or the extent of hepatic immunopathology are linked to any particular genomic intervals. The F2 mice exhibited cytokine responses and immunopathologies that revealed a statistically significant correlation between prominent egg Ag-stimulated IFN-γ production by mesenteric lymph node cells and hepatic egg granuloma size. Increased IFN-γ production showed suggestive linkage to a dominant CBA locus on chromosome 1 and a recessive CBA locus on chromosome 5; significantly, there was an epistatic interaction between the two IFN-γ loci. An additional locus with suggestive linkage to granuloma formation and a CBA-recessive mode of inheritance was mapped to centromeric chromosome 13. Our analysis identified the first three genetic regions that appear to influence the immunopathology in murine schistosomiasis; however, further congenic dissection studies will furnish a more precise understanding of the genetic control of this disease.
UR - http://www.scopus.com/inward/record.url?scp=10844261680&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=10844261680&partnerID=8YFLogxK
U2 - 10.4049/jimmunol.174.1.435
DO - 10.4049/jimmunol.174.1.435
M3 - Article
C2 - 15611268
AN - SCOPUS:10844261680
SN - 0022-1767
VL - 174
SP - 435
EP - 440
JO - Journal of Immunology
JF - Journal of Immunology
IS - 1
ER -