Enhanced endothelialization of a new stent polymer through surface enhancement and incorporation of growth factor-delivering microparticles

Hao Xu, Kytai T. Nguyen, Emmanouil S Brilakis, Jian Yang, Eric Fuh, Subhash Banerjee

Research output: Contribution to journalArticle

17 Scopus citations

Abstract

In this study, we sought to develop strategies for improved endothelialization of a new polymer coating for vascular stents. Surface enhancement of the new poly-1,8- octanediol-co-citric acid (POC) polymer was achieved through conjugation of anti-CD34 antibody and incorporation of vascular endothelial growth factor and basic fibroblast growth factor-containing poly-lactic-co-glycolic acid microparticles to improve capture and proliferation of endothelial progenitor cells (EPC) and compared to untreated POC and poly-L-lactic acid (PLLA) polymer. Our results indicate that compared to PLLA, POC coating was more hemocompatible, with less platelet activation (p=0.01), thrombogenicity (p<0.05 for 20 and 30 min clot formation), and inflammatory response (IL-1β release, p=0.0009; TNF-α release, p=0.004). EPC adhesion and proliferation on POC were significantly improved with surface enhancement and microparticle incorporation compared to untreated POC (p=0.006) and PLLA (p=0.003). These results suggest a new strategy for enhancing endothelialization of polymeric coatings of vascular prostheses.

Original languageEnglish (US)
Pages (from-to)519-527
Number of pages9
JournalJournal of cardiovascular translational research
Volume5
Issue number4
DOIs
StatePublished - Aug 1 2012

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Keywords

  • Endothelial progenitor cells
  • Endothelialization
  • Microparticle
  • Poly-1,8-octanediol-co- citric acid polymer

ASJC Scopus subject areas

  • Molecular Medicine
  • Genetics
  • Pharmaceutical Science
  • Cardiology and Cardiovascular Medicine
  • Genetics(clinical)

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