Abstract
In this study, we sought to develop strategies for improved endothelialization of a new polymer coating for vascular stents. Surface enhancement of the new poly-1,8- octanediol-co-citric acid (POC) polymer was achieved through conjugation of anti-CD34 antibody and incorporation of vascular endothelial growth factor and basic fibroblast growth factor-containing poly-lactic-co-glycolic acid microparticles to improve capture and proliferation of endothelial progenitor cells (EPC) and compared to untreated POC and poly-L-lactic acid (PLLA) polymer. Our results indicate that compared to PLLA, POC coating was more hemocompatible, with less platelet activation (p=0.01), thrombogenicity (p<0.05 for 20 and 30 min clot formation), and inflammatory response (IL-1β release, p=0.0009; TNF-α release, p=0.004). EPC adhesion and proliferation on POC were significantly improved with surface enhancement and microparticle incorporation compared to untreated POC (p=0.006) and PLLA (p=0.003). These results suggest a new strategy for enhancing endothelialization of polymeric coatings of vascular prostheses.
Original language | English (US) |
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Pages (from-to) | 519-527 |
Number of pages | 9 |
Journal | Journal of cardiovascular translational research |
Volume | 5 |
Issue number | 4 |
DOIs | |
State | Published - Aug 1 2012 |
Keywords
- Endothelial progenitor cells
- Endothelialization
- Microparticle
- Poly-1,8-octanediol-co- citric acid polymer
ASJC Scopus subject areas
- Molecular Medicine
- Genetics
- Pharmaceutical Science
- Cardiology and Cardiovascular Medicine
- Genetics(clinical)