Enhanced evolvability in immunoglobulin V genes under somatic hypermutation

Lindsay G. Cowell, Hye Jung Kim, Tiina Humaljoki, Claudia Berek, Thomas B. Kepler

Research output: Contribution to journalArticle

26 Scopus citations

Abstract

Darwinian theory requires that mutations be produced in a nonanticipatory manner; it is nonetheless consistent to suggest that mutations that have repeatedly led to nonviable phenotypes would be introduced less frequently than others-if under appropriate genetic control. Immunoglobulins produced during infection acquire point mutations that are subsequently selected for improved binding to the eliciting antigen. We and others have speculated that an enhancement of mutability in the complementarity-determining regions (CDR; where mutations have a greater chance of being advantageous) and/or decrement of mutability in the framework regions (FR; where mutations are more likely to be lethal) may be accomplished by differential codon usage in concert with the known sequence specificity of the hypermutation mechanism. We have examined 115 nonproductively rearranged human Ig sequences. The mutation patterns in these unexpressed genes are unselected and therefore directly reflect inherent mutation biases. Using a χ2 test, we have shown that the number of mutations in the CDRs is significantly higher than the number of mutations found in the FRs, providing direct evidence for the hypothesis that mutations are preferentially targeted into the CDRs.

Original languageEnglish (US)
Pages (from-to)23-26
Number of pages4
JournalJournal of Molecular Evolution
Volume49
Issue number1
DOIs
StatePublished - Jul 5 1999

Keywords

  • Adaptability
  • Affinity maturation
  • Genome evolution
  • Somatic hypermutation

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Molecular Biology
  • Genetics

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