TY - JOUR
T1 - Enhanced purification of fetal liver hematopoietic stem cells using SLAM family receptors
AU - Kim, Injune
AU - He, Shenghui
AU - Yilmaz, Ömer H.
AU - Kiel, Mark J.
AU - Morrison, Sean J.
PY - 2006/7/15
Y1 - 2006/7/15
N2 - Although adult mouse hematopoietic stem cells (HSCs) have been purified to near homogeneity, it remains impossible to achieve this with fetal HSCs. Adult HSC purity recently has been enhanced using the SLAM family receptors CD150, CD244, and CD48. These markers are expressed at different stages of the hematopoiesis hierarchy, making it possible to highly purify adult HSCs as CD150+CD48-CD244- cells. We found that SLAM family receptors exhibited a similar expression pattern in fetal liver. Fetal liver HSCs were CD150+CD48-CD244-, and the vast majority of colony-forming progenitors were CD48+CD244 -CD150- or CD48+ CD244+CD150 -, just as in adult bone marrow. SLAM family markers enhanced the purification of fetal liver HSCs. Whereas 1 (11%) of every 8.9 Thy lowSca-1+ lineage-Mac-1+ fetal liver cells gave long-term multilineage reconstitution in irradiated mice, 1 (18%) of every 5.7 CD150+ CD48-CD41- cells and 1 (37%) of every 2.7 CD150+CD48-Sca-1+lineage -Mac-1+ fetal liver cells gave long-term multilineage reconstitution. These data emphasize the robustness with which SLAM family markers distinguish progenitors at different stages of the hematopoiesis hierarchy and enhance the purification of definitive HSCs from diverse contexts. Nonetheless, CD150, CD244, and CD48 are not pan-stem cell markers, as they were not detectably expressed by stem cells in the fetal or adult nervous system.
AB - Although adult mouse hematopoietic stem cells (HSCs) have been purified to near homogeneity, it remains impossible to achieve this with fetal HSCs. Adult HSC purity recently has been enhanced using the SLAM family receptors CD150, CD244, and CD48. These markers are expressed at different stages of the hematopoiesis hierarchy, making it possible to highly purify adult HSCs as CD150+CD48-CD244- cells. We found that SLAM family receptors exhibited a similar expression pattern in fetal liver. Fetal liver HSCs were CD150+CD48-CD244-, and the vast majority of colony-forming progenitors were CD48+CD244 -CD150- or CD48+ CD244+CD150 -, just as in adult bone marrow. SLAM family markers enhanced the purification of fetal liver HSCs. Whereas 1 (11%) of every 8.9 Thy lowSca-1+ lineage-Mac-1+ fetal liver cells gave long-term multilineage reconstitution in irradiated mice, 1 (18%) of every 5.7 CD150+ CD48-CD41- cells and 1 (37%) of every 2.7 CD150+CD48-Sca-1+lineage -Mac-1+ fetal liver cells gave long-term multilineage reconstitution. These data emphasize the robustness with which SLAM family markers distinguish progenitors at different stages of the hematopoiesis hierarchy and enhance the purification of definitive HSCs from diverse contexts. Nonetheless, CD150, CD244, and CD48 are not pan-stem cell markers, as they were not detectably expressed by stem cells in the fetal or adult nervous system.
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U2 - 10.1182/blood-2005-10-4135
DO - 10.1182/blood-2005-10-4135
M3 - Article
C2 - 16569764
AN - SCOPUS:33745965645
SN - 0006-4971
VL - 108
SP - 737
EP - 744
JO - Blood
JF - Blood
IS - 2
ER -