Enhancement of cardiac L-type Ca2+ currents in transgenic mice with cardiac-specific overexpression of CYP2J2

Yong Fu Xiao, Qingen Ke, John M. Seubert, J. Alyce Bradbury, Joan Graves, Laura M. DeGraff, J R Falck, Kris Krausz, Harry V. Gelboin, James P. Morgan, Darryl C. Zeldin

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Abstract

CYP2J2 is abundant in cardiomyocytes and is involved in the metabolism of arachidonic acid (AA) to epoxyeicosatrienoic acids (EETs), which affect multiple cell functions. In this study, we investigated the effect of overexpression of CYP2J2 on cardiac L-type Ca2+ currents (ICa) in adult transgenic mice. Cardiac-specific overexpression of CYP2J2 was achieved using the α-myosin heavy chain promoter. ICa was recorded from isolated ventricular cardiomyocytes. Compared with the wild-type cardiomyocytes (n = 60), the density of ICa was significantly increased by 40 ± 9% in the CYP2J2 transgenic cardiomyocytes (n = 71; P < 0.001). N-Methylsulfonyl-6-(2-proparglyloxyphenyl)hexanamide (MS-PPOH), a specific inhibitor of EET biosynthesis, and clotrimazole, a cytochrome P450 inhibitor, significantly reduced ICa in both wild-type and transgenic cardiomyocytes; however, MS-PPOH inhibited ICa to a greater extent in the CYP2J2 transgenic cells (n = 10) than in the wild-type cells (n = 10; P < 0.01). Addition of 11,12-EET significantly restored ICa in MS-PPOH-treated cells. Intracellular dialysis with either of two inhibitory monoclonal antibodies against CYP2J2 significantly reduced ICa in both wild-type and transgenic mice. Membrane-permeable 8-bromo-cAMP and the β-adrenergic agonist isoproterenol significantly reversed the monoclonal antibody-induced inhibition of ICa. In addition, the total protein level of the α1 subunit of the Cav1.2 L-type Ca2+ channel was not altered in CYP2J2 transgenic hearts, but the phosphorylated portion was markedly increased. In conclusion, overexpression of CYP2J2 increases ICa in CYP2J2 transgenic cardiomyocytes via a mechanism that involves cAMP-protein kinase A-dependent phosphorylation of the L-type Ca2+ channel.

Original languageEnglish (US)
Pages (from-to)1607-1616
Number of pages10
JournalMolecular Pharmacology
Volume66
Issue number6
DOIs
StatePublished - Dec 2004

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Transgenic Mice
Cardiac Myocytes
Cyclic AMP-Dependent Protein Kinases
Monoclonal Antibodies
Clotrimazole
8-Bromo Cyclic Adenosine Monophosphate
arachidonate epoxygenase
Adrenergic Agonists
Myosin Heavy Chains
Isoproterenol
Arachidonic Acid
Cytochrome P-450 Enzyme System
Dialysis
Phosphorylation
Acids
Membranes
caproamide

ASJC Scopus subject areas

  • Pharmacology

Cite this

Xiao, Y. F., Ke, Q., Seubert, J. M., Bradbury, J. A., Graves, J., DeGraff, L. M., ... Zeldin, D. C. (2004). Enhancement of cardiac L-type Ca2+ currents in transgenic mice with cardiac-specific overexpression of CYP2J2. Molecular Pharmacology, 66(6), 1607-1616. https://doi.org/10.1124/mol.104.004150

Enhancement of cardiac L-type Ca2+ currents in transgenic mice with cardiac-specific overexpression of CYP2J2. / Xiao, Yong Fu; Ke, Qingen; Seubert, John M.; Bradbury, J. Alyce; Graves, Joan; DeGraff, Laura M.; Falck, J R; Krausz, Kris; Gelboin, Harry V.; Morgan, James P.; Zeldin, Darryl C.

In: Molecular Pharmacology, Vol. 66, No. 6, 12.2004, p. 1607-1616.

Research output: Contribution to journalArticle

Xiao, YF, Ke, Q, Seubert, JM, Bradbury, JA, Graves, J, DeGraff, LM, Falck, JR, Krausz, K, Gelboin, HV, Morgan, JP & Zeldin, DC 2004, 'Enhancement of cardiac L-type Ca2+ currents in transgenic mice with cardiac-specific overexpression of CYP2J2', Molecular Pharmacology, vol. 66, no. 6, pp. 1607-1616. https://doi.org/10.1124/mol.104.004150
Xiao, Yong Fu ; Ke, Qingen ; Seubert, John M. ; Bradbury, J. Alyce ; Graves, Joan ; DeGraff, Laura M. ; Falck, J R ; Krausz, Kris ; Gelboin, Harry V. ; Morgan, James P. ; Zeldin, Darryl C. / Enhancement of cardiac L-type Ca2+ currents in transgenic mice with cardiac-specific overexpression of CYP2J2. In: Molecular Pharmacology. 2004 ; Vol. 66, No. 6. pp. 1607-1616.
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AU - Seubert, John M.

AU - Bradbury, J. Alyce

AU - Graves, Joan

AU - DeGraff, Laura M.

AU - Falck, J R

AU - Krausz, Kris

AU - Gelboin, Harry V.

AU - Morgan, James P.

AU - Zeldin, Darryl C.

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