Enhancement of fluorinated pyrimidine-induced cytotoxicity by leucovorin in human lung cancer cell lines

C. M. Tsai, A. F. Gazdar, C. Allegra, R. P. Perng, B. S. Kramer

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Reduced folates have been shown to increase the cytotoxicity of 5-fluorouracil (FUra) by stabilizing the fluorodexoyuridine monophosphate:thymidylate synthase complex, thus increasing the block in the DNA synthetic pathway. Using an in vitro tetrazolium colorimetric (MTT) cytotoxic assay, we tested the effects of FUra and 5-fluorodeoxyuridine (FUdR) with and without leucovorin (LV) on a panel of 7 human lung cancer cell lines. LV at a concentration of 20 μM enhanced the cytotoxicity of FUra and of FUdR in all of the cell lines. Quantitatively, LV had a higher degree of enhancement on FUdR than on FUra cytotoxicity in 6 cell lines. There was equivalent enhancement in the only remaining line. The differential effects of LV on the cytotoxicity of FUra vs FUdR in these lung carcinoma lines contrasts with a quantitatively similar enhancement of cytotoxicity between FUra and FUdR in colon cancer lines previously reported from our laboratory. This suggests that the metabolism of FUra may be different in these lung cancer cell lines.

Original languageEnglish (US)
Pages (from-to)101-105
Number of pages5
JournalInternational Journal of Cancer
Volume46
Issue number1
DOIs
StatePublished - 1990

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Leucovorin
Floxuridine
Fluorouracil
Lung Neoplasms
Cell Line
Thymidylate Synthase
pyrimidine
Folic Acid
Colonic Neoplasms
Carcinoma
Lung
DNA

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Enhancement of fluorinated pyrimidine-induced cytotoxicity by leucovorin in human lung cancer cell lines. / Tsai, C. M.; Gazdar, A. F.; Allegra, C.; Perng, R. P.; Kramer, B. S.

In: International Journal of Cancer, Vol. 46, No. 1, 1990, p. 101-105.

Research output: Contribution to journalArticle

Tsai, C. M. ; Gazdar, A. F. ; Allegra, C. ; Perng, R. P. ; Kramer, B. S. / Enhancement of fluorinated pyrimidine-induced cytotoxicity by leucovorin in human lung cancer cell lines. In: International Journal of Cancer. 1990 ; Vol. 46, No. 1. pp. 101-105.
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