Enhancement of strand invasion by oligonucleotides through manipulation of backbone charge

Sergey V. Smulevitch, Carla G. Simmons, James C. Norton, Teresa W. Wise, David R. Corey

Research output: Contribution to journalArticle

64 Scopus citations

Abstract

The ability of DNA oligonucleotides, neutral peptide nucleic acids (PNAs), and oligonucleotide conjugates to hybridize to inverted repeat sequences within supercolled double-stranded DNA by Watson-Crick base-pairing is examined. PNAs and oligonucleotide conjugates initiate and maintain strand invasion under more stringent conditions than do unmodified DNA oligonucleotides. PNAs hybridize rapidly and, once bound, hold open a target site allowing oligonucleotides to base-pair to the displaced strand under conditions that would otherwise preclude hybridization. The ability to manipulate hybridization efficiency through different options for the alteration of oligomer charge should have important implications for optimizing sequence-specific recognition of DNA.

Original languageEnglish (US)
Pages (from-to)1700-1704
Number of pages5
JournalNature biotechnology
Volume14
Issue number13
DOIs
StatePublished - Dec 1996

Keywords

  • Macromolecular engineering
  • Oligonucleotide
  • Peptide nucleic acid
  • Strand invasion

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology
  • Molecular Medicine
  • Biomedical Engineering

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