Enhancing contrast and quantitation by spatial frequency domain fluorescence molecular imaging

Jessica Sun, Deep Hathi, Haiying Zhou, Monica Shokeen, Walter J. Akers

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Scopus citations


Optical imaging with fluorescent contrast agents is highly sensitive for molecular imaging but is limited in depth to a few centimeters below the skin. Planar fluorescence imaging with full-field, uniform illumination and scientific camera image capture provides a portable and robust configuration for real-time, sensitive fluorescence detection with scalable resolution, but is inherently surface weighted and therefore limited in depth to a few millimeters. At the NIR region (700-1000 nm), tissue absorption and autofluorescence are relatively reduced, increasing depth penetration and reducing background signal, respectively. Optical imaging resolution scales with depth, limiting microscopic resolution with multiphoton microscopy and optical coherence tomography to < 3 mm depth. Unfortunately, patient skin and peri-tumoral tissues are not uniform, varying in thickness and color, complicating subsurface fluorescence measurements. Diffuse optical imaging methods have been developed that better quantify optical signals relative to faster full-field planar reflectance imaging, but require long scan times, complex instrumentation, and reconstruction algorithms. Here we report a novel strategy for rapid measurement of subsurface fluorescence using structured light illumination to improve quantitation of deep-seated fluorescence molecular probe accumulation. This technique, in combination with highly specific, tumor-avid fluorescent molecular probes, will easily integrate noninvasive diagnostics for superficial cancers and fluorescence guided surgery.

Original languageEnglish (US)
Title of host publicationOptical Diagnostics and Sensing XVI
Subtitle of host publicationToward Point-of-Care Diagnostics
EditorsGerard L. Cote
ISBN (Electronic)9781628419498
StatePublished - 2016
Externally publishedYes
EventOptical Diagnostics and Sensing XVI: Toward Point-of-Care Diagnostics - San Francisco, United States
Duration: Feb 15 2016Feb 16 2016

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
ISSN (Print)1605-7422


ConferenceOptical Diagnostics and Sensing XVI: Toward Point-of-Care Diagnostics
Country/TerritoryUnited States
CitySan Francisco


  • biomedical imaging
  • cancer
  • diffuse optics
  • imaging systems
  • molecular imaging
  • spatial frequency domain
  • xenograft

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Atomic and Molecular Physics, and Optics
  • Radiology Nuclear Medicine and imaging


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