Enhancing hepatocyte adhesion by pulsed plasma deposition and polyethylene glycol coupling

Eric S. Carlisle, Muthumarthanda R. Mariappan, Kevin D. Nelson, Brett E. Thomes, Richard B. Timmons, Anca Constantinescu, Robert C. Eberhart, Paul E. Bankey

Research output: Contribution to journalArticle

89 Citations (Scopus)

Abstract

Decreased hepatocyte adhesion to polymeric constructs limits the function of tissue engineered hepatic assist devices. We grafted adhesion peptides (RGD and YIGSR) to polycaprolactone (PCL) and poly-L-lactic acid (PLLA) in order to mimic the in vivo extracellular matrix and thus enhance hepatocyte adhesion. Peptide grafting was done by a novel technique in which polyethylene glycol (PEG)-adhesion peptide was linked to allyl-amine coated on the surface of PCL and PLLA by pulsed plasma deposition (PPD). Peptide grafting density, quantified by radio-iodinated tyrosine in YIGSR, was 158 fmol/cm2 on PLLA and 425 fmol/cm2 on PCL surfaces. The adhesion of hepatocytes was determined by plating 250,000 hepatocytes/well (test substrates were coated on 12 well plates) and quantifying the percentage of adhered cells after 6 h by MTT assay. Adhesion on PCL surfaces was significantly enhanced (p < 0.05) by both YIGSR (percentage of adhered cells = 53 ± 7%) and RGD (53 ± 12%) when compared to control surfaces (31 ± 8%). Hepatocyte adhesion on PLLA was significantly (p < 0.05) enhanced on PLLA- PEG-RGD surfaces (76 ± 14%) compared to control surfaces (42 ± 19%) and more (68 ± 25%) but not statistically significant (p = 0.15) on PLLA-PEG- YIGSR surfaces compared to control surfaces. These results indicate that hepatocyte adhesion to PCL and PLLA based polymeric surfaces can be enhanced by a novel adhesion peptide grafting technique using pulsed plasma deposition and PEG cross-linking.

Original languageEnglish (US)
Pages (from-to)45-52
Number of pages8
JournalTissue Engineering
Volume6
Issue number1
DOIs
StatePublished - Feb 2000

Fingerprint

Plasma deposition
tyrosyl-isoleucyl-glycyl-seryl-arginine
Polyethylene glycols
Hepatocytes
Lactic acid
Adhesion
Polycaprolactone
Peptides
Control surfaces
poly(lactic acid)
Radio
Amines
Extracellular Matrix
Tyrosine
Plating
polycaprolactone
Assays
Equipment and Supplies
Liver
Tissue

ASJC Scopus subject areas

  • Biophysics
  • Cell Biology

Cite this

Carlisle, E. S., Mariappan, M. R., Nelson, K. D., Thomes, B. E., Timmons, R. B., Constantinescu, A., ... Bankey, P. E. (2000). Enhancing hepatocyte adhesion by pulsed plasma deposition and polyethylene glycol coupling. Tissue Engineering, 6(1), 45-52. https://doi.org/10.1089/107632700320883

Enhancing hepatocyte adhesion by pulsed plasma deposition and polyethylene glycol coupling. / Carlisle, Eric S.; Mariappan, Muthumarthanda R.; Nelson, Kevin D.; Thomes, Brett E.; Timmons, Richard B.; Constantinescu, Anca; Eberhart, Robert C.; Bankey, Paul E.

In: Tissue Engineering, Vol. 6, No. 1, 02.2000, p. 45-52.

Research output: Contribution to journalArticle

Carlisle, ES, Mariappan, MR, Nelson, KD, Thomes, BE, Timmons, RB, Constantinescu, A, Eberhart, RC & Bankey, PE 2000, 'Enhancing hepatocyte adhesion by pulsed plasma deposition and polyethylene glycol coupling', Tissue Engineering, vol. 6, no. 1, pp. 45-52. https://doi.org/10.1089/107632700320883
Carlisle ES, Mariappan MR, Nelson KD, Thomes BE, Timmons RB, Constantinescu A et al. Enhancing hepatocyte adhesion by pulsed plasma deposition and polyethylene glycol coupling. Tissue Engineering. 2000 Feb;6(1):45-52. https://doi.org/10.1089/107632700320883
Carlisle, Eric S. ; Mariappan, Muthumarthanda R. ; Nelson, Kevin D. ; Thomes, Brett E. ; Timmons, Richard B. ; Constantinescu, Anca ; Eberhart, Robert C. ; Bankey, Paul E. / Enhancing hepatocyte adhesion by pulsed plasma deposition and polyethylene glycol coupling. In: Tissue Engineering. 2000 ; Vol. 6, No. 1. pp. 45-52.
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