Enteroid Monolayers Reveal an Autonomous WNT and BMP Circuit Controlling Intestinal Epithelial Growth and Organization

Curtis A. Thorne, Ina W. Chen, Laura E. Sanman, Melanie H. Cobb, Lani F. Wu, Steven J. Altschuler

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

The intestinal epithelium maintains a remarkable balance between proliferation and differentiation despite rapid cellular turnover. A central challenge is to elucidate mechanisms required for robust control of tissue renewal. Opposing WNT and BMP signaling is essential in establishing epithelial homeostasis. However, it has been difficult to disentangle contributions from multiple sources of morphogen signals in the tissue. Here, to dissect epithelial-autonomous morphogenic signaling circuits, we developed an enteroid monolayer culture system that recapitulates four key properties of the intestinal epithelium, namely the ability to maintain proliferative and differentiated zones, self-renew, polarize, and generate major intestinal cell types. We systematically perturb intrinsic and extrinsic sources of WNT and BMP signals to reveal a core morphogenic circuit that controls proliferation, tissue organization, and cell fate. Our work demonstrates the ability of intestinal epithelium, even in the absence of 3D tissue architecture, to control its own growth and organization through morphogen-mediated feedback. Thorne, Chen et al. develop an enteroid monolayer culture system that recapitulates cell-type composition, crypt organization, and turnover kinetics of the intestinal epithelium. The authors use the enteroid monolayer to uncover an epithelium-intrinsic Wnt and BMP morphogenic circuit that regulates proliferation and tissue organization in the intestinal epithelium.

Original languageEnglish (US)
Pages (from-to)624-633.e4
JournalDevelopmental Cell
Volume44
Issue number5
DOIs
StatePublished - Mar 12 2018

Fingerprint

Intestinal Mucosa
Monolayers
Tissue
Networks (circuits)
Growth
Robust control
Homeostasis
Epithelium
Feedback
Kinetics
Chemical analysis

Keywords

  • BMP
  • crypt
  • epithelium
  • feedback
  • homeostasis
  • intestine
  • organoid
  • proliferation
  • Wnt

ASJC Scopus subject areas

  • Developmental Biology

Cite this

Enteroid Monolayers Reveal an Autonomous WNT and BMP Circuit Controlling Intestinal Epithelial Growth and Organization. / Thorne, Curtis A.; Chen, Ina W.; Sanman, Laura E.; Cobb, Melanie H.; Wu, Lani F.; Altschuler, Steven J.

In: Developmental Cell, Vol. 44, No. 5, 12.03.2018, p. 624-633.e4.

Research output: Contribution to journalArticle

Thorne, Curtis A. ; Chen, Ina W. ; Sanman, Laura E. ; Cobb, Melanie H. ; Wu, Lani F. ; Altschuler, Steven J. / Enteroid Monolayers Reveal an Autonomous WNT and BMP Circuit Controlling Intestinal Epithelial Growth and Organization. In: Developmental Cell. 2018 ; Vol. 44, No. 5. pp. 624-633.e4.
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