TY - JOUR
T1 - ENU mutagenesis in mice.
AU - Georgel, Philippe
AU - Du, Xin
AU - Hoebe, Kasper
AU - Beutler, Bruce
PY - 2008
Y1 - 2008
N2 - Forward genetics has led to many "breakthrough" discoveries, and with the mouse genome almost fully sequenced, the creation of phenotypes through random germline mutagenesis has become an efficient means by which to find the function of yet undescribed genes. In this chapter, we will provide a practical guideline for performing germline mutagenesis in mice. In particular, we will focus on the application of this technology to identify genes that are essential to innate immune defense.
AB - Forward genetics has led to many "breakthrough" discoveries, and with the mouse genome almost fully sequenced, the creation of phenotypes through random germline mutagenesis has become an efficient means by which to find the function of yet undescribed genes. In this chapter, we will provide a practical guideline for performing germline mutagenesis in mice. In particular, we will focus on the application of this technology to identify genes that are essential to innate immune defense.
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U2 - 10.1007/978-1-59745-570-1_1
DO - 10.1007/978-1-59745-570-1_1
M3 - Article
C2 - 18370145
AN - SCOPUS:45549091153
SN - 1064-3745
VL - 415
SP - 1
EP - 16
JO - Methods in molecular biology (Clifton, N.J.)
JF - Methods in molecular biology (Clifton, N.J.)
ER -