ENU mutagenesis in mice.

Philippe Georgel, Xin Du, Kasper Hoebe, Bruce Beutler

Research output: Contribution to journalArticle

38 Citations (Scopus)

Abstract

Forward genetics has led to many "breakthrough" discoveries, and with the mouse genome almost fully sequenced, the creation of phenotypes through random germline mutagenesis has become an efficient means by which to find the function of yet undescribed genes. In this chapter, we will provide a practical guideline for performing germline mutagenesis in mice. In particular, we will focus on the application of this technology to identify genes that are essential to innate immune defense.

Original languageEnglish (US)
Pages (from-to)1-16
Number of pages16
JournalMethods in molecular biology (Clifton, N.J.)
Volume415
StatePublished - 2008

Fingerprint

Mutagenesis
Essential Genes
Genome
Guidelines
Technology
Phenotype
Genes

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

ENU mutagenesis in mice. / Georgel, Philippe; Du, Xin; Hoebe, Kasper; Beutler, Bruce.

In: Methods in molecular biology (Clifton, N.J.), Vol. 415, 2008, p. 1-16.

Research output: Contribution to journalArticle

Georgel, P, Du, X, Hoebe, K & Beutler, B 2008, 'ENU mutagenesis in mice.', Methods in molecular biology (Clifton, N.J.), vol. 415, pp. 1-16.
Georgel, Philippe ; Du, Xin ; Hoebe, Kasper ; Beutler, Bruce. / ENU mutagenesis in mice. In: Methods in molecular biology (Clifton, N.J.). 2008 ; Vol. 415. pp. 1-16.
@article{d807176eb5ea4f4c8ca6b23cdc5b8540,
title = "ENU mutagenesis in mice.",
abstract = "Forward genetics has led to many {"}breakthrough{"} discoveries, and with the mouse genome almost fully sequenced, the creation of phenotypes through random germline mutagenesis has become an efficient means by which to find the function of yet undescribed genes. In this chapter, we will provide a practical guideline for performing germline mutagenesis in mice. In particular, we will focus on the application of this technology to identify genes that are essential to innate immune defense.",
author = "Philippe Georgel and Xin Du and Kasper Hoebe and Bruce Beutler",
year = "2008",
language = "English (US)",
volume = "415",
pages = "1--16",
journal = "Methods in molecular biology (Clifton, N.J.)",
issn = "1064-3745",
publisher = "Humana Press",

}

TY - JOUR

T1 - ENU mutagenesis in mice.

AU - Georgel, Philippe

AU - Du, Xin

AU - Hoebe, Kasper

AU - Beutler, Bruce

PY - 2008

Y1 - 2008

N2 - Forward genetics has led to many "breakthrough" discoveries, and with the mouse genome almost fully sequenced, the creation of phenotypes through random germline mutagenesis has become an efficient means by which to find the function of yet undescribed genes. In this chapter, we will provide a practical guideline for performing germline mutagenesis in mice. In particular, we will focus on the application of this technology to identify genes that are essential to innate immune defense.

AB - Forward genetics has led to many "breakthrough" discoveries, and with the mouse genome almost fully sequenced, the creation of phenotypes through random germline mutagenesis has become an efficient means by which to find the function of yet undescribed genes. In this chapter, we will provide a practical guideline for performing germline mutagenesis in mice. In particular, we will focus on the application of this technology to identify genes that are essential to innate immune defense.

UR - http://www.scopus.com/inward/record.url?scp=45549091153&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=45549091153&partnerID=8YFLogxK

M3 - Article

VL - 415

SP - 1

EP - 16

JO - Methods in molecular biology (Clifton, N.J.)

JF - Methods in molecular biology (Clifton, N.J.)

SN - 1064-3745

ER -