Enucleation of cultural human cells

W. E. Wright, L. Hayflick

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Human cells were found to have a biphasic response to high g force enucleation in the presence of cytochalasin B (CB). At higher g forces, enucleation is exquisitely CB dependent. At lower g forces, enucleation becomes independent of the concentration of CB and highly dependent upon temperature. Because of the ability to manipulate this part of the response with temperature, glutaraldehyde and amphotericin B, it is suggested that at lower g forces the rigidity of the cell membrane becomes limiting for enucleation. Cells that are sensitive to enucleation at 1g would thus be sensitive because of a difference in the physical characteristics of their membranes, rather than a different sensitivity of their receptors to CB itself. The processes involved in enucleation were found to exhibit multihit kinetics, and were not specific for the nucleus. A model based on the ability of CB to disrupt a subplasmalemmal network of microfilaments is presented to explain these observations.

Original languageEnglish (US)
Pages (from-to)587-592
Number of pages6
JournalProceedings of the Society for Experimental Biology and Medicine
Volume144
Issue number2
StatePublished - 1973

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Cytochalasin B
Cells
Temperature
Glutaral
Amphotericin B
Cell membranes
Actin Cytoskeleton
Rigidity
Cell Membrane
Membranes
Kinetics

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Enucleation of cultural human cells. / Wright, W. E.; Hayflick, L.

In: Proceedings of the Society for Experimental Biology and Medicine, Vol. 144, No. 2, 1973, p. 587-592.

Research output: Contribution to journalArticle

Wright, WE & Hayflick, L 1973, 'Enucleation of cultural human cells', Proceedings of the Society for Experimental Biology and Medicine, vol. 144, no. 2, pp. 587-592.
Wright, W. E. ; Hayflick, L. / Enucleation of cultural human cells. In: Proceedings of the Society for Experimental Biology and Medicine. 1973 ; Vol. 144, No. 2. pp. 587-592.
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