Enumeration of Plasmacytoid Dendritic Cells in Peripheral Blood and Bone Marrow by Flow-Cytometric Analysis

Abdullah Alsuwaidan, Franklin Fuda, Weina Chen, Mingyi Chen

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Dendritic cells (DCs) are powerful antigen presenting cells that are involved in regulating immune response. Plasmacytoid dendritic cells (pDCs) are subtype of DCs that present in small quantity in the bone marrow, peripheral blood, and lymph nodes. They are important component of our immune system in normal condition and diseases. They activate T cells and play a critical role in immune tolerance. In this chapter we review the immunophenotypic features of pDCs and provide a practical protocol for pDCs enumeration in the peripheral blood and bone marrow samples.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages193-201
Number of pages9
DOIs
StatePublished - Jan 1 2019

Publication series

NameMethods in Molecular Biology
Volume2032
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

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Keywords

  • Dendritic cells
  • Flow cytometry
  • Immunophenotyping
  • Plasmacytoid dendritic cells

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Alsuwaidan, A., Fuda, F., Chen, W., & Chen, M. (2019). Enumeration of Plasmacytoid Dendritic Cells in Peripheral Blood and Bone Marrow by Flow-Cytometric Analysis. In Methods in Molecular Biology (pp. 193-201). (Methods in Molecular Biology; Vol. 2032). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-9650-6_11