Enumeration of Plasmacytoid Dendritic Cells in Peripheral Blood and Bone Marrow by Flow-Cytometric Analysis

Abdullah Alsuwaidan, Franklin S Fuda, Weina Chen, Mingyi Chen

Research output: Contribution to journalArticle

Abstract

Dendritic cells (DCs) are powerful antigen presenting cells that are involved in regulating immune response. Plasmacytoid dendritic cells (pDCs) are subtype of DCs that present in small quantity in the bone marrow, peripheral blood, and lymph nodes. They are important component of our immune system in normal condition and diseases. They activate T cells and play a critical role in immune tolerance. In this chapter we review the immunophenotypic features of pDCs and provide a practical protocol for pDCs enumeration in the peripheral blood and bone marrow samples.

Original languageEnglish (US)
Pages (from-to)193-201
Number of pages9
JournalMethods in molecular biology (Clifton, N.J.)
Volume2032
DOIs
StatePublished - Jan 1 2019

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Dendritic Cells
Bone Marrow
Immune Tolerance
Antigen-Presenting Cells
Immune System
Lymph Nodes
T-Lymphocytes

Keywords

  • Dendritic cells
  • Flow cytometry
  • Immunophenotyping
  • Plasmacytoid dendritic cells

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

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abstract = "Dendritic cells (DCs) are powerful antigen presenting cells that are involved in regulating immune response. Plasmacytoid dendritic cells (pDCs) are subtype of DCs that present in small quantity in the bone marrow, peripheral blood, and lymph nodes. They are important component of our immune system in normal condition and diseases. They activate T cells and play a critical role in immune tolerance. In this chapter we review the immunophenotypic features of pDCs and provide a practical protocol for pDCs enumeration in the peripheral blood and bone marrow samples.",
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