Enzymatic assay of Dmannose from urine

Iti Mehta, Philippe E Zimmern, Larry Reitzer

Research output: Contribution to journalArticle

1 Scopus citations

Abstract

Aim: Urinary tract infections (UTIs) are increasingly antibiotic resistant, and alternate or adjunct therapies are urgently needed. Several studies suggest that D mannose ingestion and a hypothesized increase in urinary Dmannose reduce UTI frequency. Our goal was to develop a reliable assay for urinary Dmannose, which is needed to assess the effects of supplemental Dmannose on urinary Dmannose and UTIs. Results: We developed an enzymatic assay for Dmannose in urine. Hexoses in urine were phosphorylated, sequentially isomerized and oxidized, and the increases in reduced NADPH were measured in a spectrophotometer. Urinary mannose from ten volunteers was well above the detection limit and ranged from 8 to 700 μM. Conclusion: A rapid, reliable, and sensitive assay was developed, readily detected urinary Dmannose, and is adaptable to highthroughput analysis. If urinary Dmannose is shown to correlate with susceptibility to UTIs, then the assay could assess susceptibility to UTIs and direct mannose therapy.

Original languageEnglish (US)
Pages (from-to)1947-1954
Number of pages8
JournalBioanalysis
Volume10
Issue number23
DOIs
Publication statusPublished - Dec 1 2018

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Keywords

  • enzymatic analysis
  • mannose
  • methodology
  • urinary tract infections
  • urine

ASJC Scopus subject areas

  • Analytical Chemistry
  • Pharmacology, Toxicology and Pharmaceutics(all)
  • Clinical Biochemistry
  • Medical Laboratory Technology

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