Abstract
Further studies have confirmed earlier observation that in the presence of EDTA, degradation of phage BPS2 [3H] uracil labeled DNA is effected by an N glycosidase activity in extracts of Bacillus subtilis that removes free uracil from DNA. In addition, such extracts contain a nuclease activity that attacks PBS2 DNA in the presence of CaCl2. The nuclease activity is not observed under conditions that inactivate N glycosidase activity but does attack DNA that has been preincubated to remove uracil by N glycosidase action. The authors therefore postulate that the nuclease requires N glycosidase action to generate substrate for its activity, i.e., the nuclease appears to attack depyrimidinated sites rather than uracil sites in phage PBS2 DNA.
Original language | English (US) |
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Pages (from-to) | 338-345 |
Number of pages | 8 |
Journal | Journal of virology |
Volume | 19 |
Issue number | 2 |
DOIs | |
State | Published - 1976 |
ASJC Scopus subject areas
- Microbiology
- Immunology
- Insect Science
- Virology