Enzymatic measurement of free and esterified cholesterol levels in plasma and other biological preparations using the oxygen electrode in a modified glucose analyzer

J. M. Dietschy; L. E. Weeks; J. J. Delente

doi:10.1016/0009-8981(76)90141-8

Enzymatic measurement of free and esterified cholesterol levels in plasma and other biological preparations using the oxygen electrode in a modified glucose analyzer

J. M. Dietschy, L. E. Weeks, J. J. Delente

Internal Medicine

Research output: Contribution to journal › Article › peer-review

19 Scopus citations

Abstract

A method is described for assaying free and esterified cholesterol using the oxygen electrode in a modified glucose analyzer to measure the relative amount of oxygen utilization taking place during oxydation of free cholesterol by the enzyme, cholesterol oxidase. A second enzyme, cholesterol ester hydrolase, is utilized to generate free cholesterol from cholesterol esters. This assay procedure is rapid, specific, reproducible and applicable to the measurement of free and esterified cholesterol carried in the major plasma lipoprotein fractions of man and the rat and, in addition, it can be utilized for the assay of sterols in subcellular fractions of cells.

Original language	English (US)
Pages (from-to)	407-414
Number of pages	8
Journal	Clinica Chimica Acta
Volume	73
Issue number	3
DOIs	https://doi.org/10.1016/0009-8981(76)90141-8
State	Published - Dec 1976

ASJC Scopus subject areas

Biochemistry
Clinical Biochemistry
Biochemistry, medical

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10.1016/0009-8981(76)90141-8

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title = "Enzymatic measurement of free and esterified cholesterol levels in plasma and other biological preparations using the oxygen electrode in a modified glucose analyzer",

abstract = "A method is described for assaying free and esterified cholesterol using the oxygen electrode in a modified glucose analyzer to measure the relative amount of oxygen utilization taking place during oxydation of free cholesterol by the enzyme, cholesterol oxidase. A second enzyme, cholesterol ester hydrolase, is utilized to generate free cholesterol from cholesterol esters. This assay procedure is rapid, specific, reproducible and applicable to the measurement of free and esterified cholesterol carried in the major plasma lipoprotein fractions of man and the rat and, in addition, it can be utilized for the assay of sterols in subcellular fractions of cells.",

author = "Dietschy, {J. M.} and Weeks, {L. E.} and Delente, {J. J.}",

note = "Funding Information: This work was supported by a grant from the Monsanto Company. Dr. Diet-schy is also supported by U.S. Public Health Service Research Grants Nos. HL09610 and AM16386 and by U.S. Public Health Service Training Grant GM00034.",

year = "1976",

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doi = "10.1016/0009-8981(76)90141-8",

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T1 - Enzymatic measurement of free and esterified cholesterol levels in plasma and other biological preparations using the oxygen electrode in a modified glucose analyzer

AU - Dietschy, J. M.

AU - Weeks, L. E.

AU - Delente, J. J.

N1 - Funding Information: This work was supported by a grant from the Monsanto Company. Dr. Diet-schy is also supported by U.S. Public Health Service Research Grants Nos. HL09610 and AM16386 and by U.S. Public Health Service Training Grant GM00034.

PY - 1976/12

Y1 - 1976/12

N2 - A method is described for assaying free and esterified cholesterol using the oxygen electrode in a modified glucose analyzer to measure the relative amount of oxygen utilization taking place during oxydation of free cholesterol by the enzyme, cholesterol oxidase. A second enzyme, cholesterol ester hydrolase, is utilized to generate free cholesterol from cholesterol esters. This assay procedure is rapid, specific, reproducible and applicable to the measurement of free and esterified cholesterol carried in the major plasma lipoprotein fractions of man and the rat and, in addition, it can be utilized for the assay of sterols in subcellular fractions of cells.

AB - A method is described for assaying free and esterified cholesterol using the oxygen electrode in a modified glucose analyzer to measure the relative amount of oxygen utilization taking place during oxydation of free cholesterol by the enzyme, cholesterol oxidase. A second enzyme, cholesterol ester hydrolase, is utilized to generate free cholesterol from cholesterol esters. This assay procedure is rapid, specific, reproducible and applicable to the measurement of free and esterified cholesterol carried in the major plasma lipoprotein fractions of man and the rat and, in addition, it can be utilized for the assay of sterols in subcellular fractions of cells.

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Enzymatic measurement of free and esterified cholesterol levels in plasma and other biological preparations using the oxygen electrode in a modified glucose analyzer

Abstract

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