Enzymatic preparation of radiolabeled succinic semialdehyde

Kenneth M. Gibson; Lawrence Sweetman

doi:10.1016/0003-2697(83)90707-8

Enzymatic preparation of radiolabeled succinic semialdehyde

Kenneth M. Gibson, Lawrence Sweetman

Research output: Contribution to journal › Article › peer-review

7 Scopus citations

Abstract

[U-¹⁴C]Succinic semialdehyde was prepared with yields of 30-40% by oxidation of purified [U-¹⁴C]4-aminobutyric acid with commercially available bovine plasma monoamine oxidase. [U-¹⁴C]Succinic semialdehyde was purified by cation-exchange chromatography and quantified as the oxime and methoxime derivatives using liquid partition chromatography on silicic acid. The availability of [U-¹⁴C]succinic semialdehyde permits the reliable assay of succinic semialdehyde dehydrogenase in crude cell extracts of lymphocytes isolated from human blood, cultured human lymphoblasts, and other tissues where 4-aminobutyric acid metabolism is known to occur.

Original language	English (US)
Pages (from-to)	436-437
Number of pages	2
Journal	Analytical biochemistry
Volume	135
Issue number	2
DOIs	https://doi.org/10.1016/0003-2697(83)90707-8
State	Published - Dec 1983
Externally published	Yes

Keywords

GABA
lymphoblast
lymphocyte
monoamine oxidase
succinic semialdehyde
succinic semialdehyde dehydrogenase

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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10.1016/0003-2697(83)90707-8

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@article{3913202459f542c09a5e90f7af667a34,

title = "Enzymatic preparation of radiolabeled succinic semialdehyde",

abstract = "[U-14C]Succinic semialdehyde was prepared with yields of 30-40% by oxidation of purified [U-14C]4-aminobutyric acid with commercially available bovine plasma monoamine oxidase. [U-14C]Succinic semialdehyde was purified by cation-exchange chromatography and quantified as the oxime and methoxime derivatives using liquid partition chromatography on silicic acid. The availability of [U-14C]succinic semialdehyde permits the reliable assay of succinic semialdehyde dehydrogenase in crude cell extracts of lymphocytes isolated from human blood, cultured human lymphoblasts, and other tissues where 4-aminobutyric acid metabolism is known to occur.",

keywords = "GABA, lymphoblast, lymphocyte, monoamine oxidase, succinic semialdehyde, succinic semialdehyde dehydrogenase",

author = "Gibson, {Kenneth M.} and Lawrence Sweetman",

note = "Funding Information: {\textquoteright} This work was supported in part by U. S. Public Health Services Grant No. HD04608 from the National Institute of Child Health and Human Development, and Grant No. GM-17702 from the National Institute of General Medical Sciences, National Institutes of Health. * Abbreviations used: SSA, succinic semialdehyde; GABA, 4-aminobutyric acid, SSADH, succinic semialdehyde dehydrogenase; LPC, liquid partition chromatography. Copyright: Copyright 2014 Elsevier B.V., All rights reserved.",

year = "1983",

month = dec,

doi = "10.1016/0003-2697(83)90707-8",

language = "English (US)",

volume = "135",

pages = "436--437",

journal = "Analytical biochemistry",

issn = "0003-2697",

publisher = "Academic Press Inc.",

number = "2",

}

TY - JOUR

T1 - Enzymatic preparation of radiolabeled succinic semialdehyde

AU - Gibson, Kenneth M.

AU - Sweetman, Lawrence

N1 - Funding Information: ’ This work was supported in part by U. S. Public Health Services Grant No. HD04608 from the National Institute of Child Health and Human Development, and Grant No. GM-17702 from the National Institute of General Medical Sciences, National Institutes of Health. * Abbreviations used: SSA, succinic semialdehyde; GABA, 4-aminobutyric acid, SSADH, succinic semialdehyde dehydrogenase; LPC, liquid partition chromatography. Copyright: Copyright 2014 Elsevier B.V., All rights reserved.

PY - 1983/12

Y1 - 1983/12

N2 - [U-14C]Succinic semialdehyde was prepared with yields of 30-40% by oxidation of purified [U-14C]4-aminobutyric acid with commercially available bovine plasma monoamine oxidase. [U-14C]Succinic semialdehyde was purified by cation-exchange chromatography and quantified as the oxime and methoxime derivatives using liquid partition chromatography on silicic acid. The availability of [U-14C]succinic semialdehyde permits the reliable assay of succinic semialdehyde dehydrogenase in crude cell extracts of lymphocytes isolated from human blood, cultured human lymphoblasts, and other tissues where 4-aminobutyric acid metabolism is known to occur.

AB - [U-14C]Succinic semialdehyde was prepared with yields of 30-40% by oxidation of purified [U-14C]4-aminobutyric acid with commercially available bovine plasma monoamine oxidase. [U-14C]Succinic semialdehyde was purified by cation-exchange chromatography and quantified as the oxime and methoxime derivatives using liquid partition chromatography on silicic acid. The availability of [U-14C]succinic semialdehyde permits the reliable assay of succinic semialdehyde dehydrogenase in crude cell extracts of lymphocytes isolated from human blood, cultured human lymphoblasts, and other tissues where 4-aminobutyric acid metabolism is known to occur.

KW - GABA

KW - lymphoblast

KW - lymphocyte

KW - monoamine oxidase

KW - succinic semialdehyde

KW - succinic semialdehyde dehydrogenase

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U2 - 10.1016/0003-2697(83)90707-8

DO - 10.1016/0003-2697(83)90707-8

M3 - Article

C2 - 6660517

AN - SCOPUS:0020986786

SN - 0003-2697

VL - 135

SP - 436

EP - 437

JO - Analytical biochemistry

JF - Analytical biochemistry

IS - 2

ER -

Enzymatic preparation of radiolabeled succinic semialdehyde

Abstract

Keywords

ASJC Scopus subject areas

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