Enzymatic preparation of radiolabeled succinic semialdehyde

Kenneth M. Gibson, Lawrence Sweetman

Research output: Contribution to journalArticlepeer-review

Abstract

[U-14C]Succinic semialdehyde was prepared with yields of 30-40% by oxidation of purified [U-14C]4-aminobutyric acid with commercially available bovine plasma monoamine oxidase. [U-14C]Succinic semialdehyde was purified by cation-exchange chromatography and quantified as the oxime and methoxime derivatives using liquid partition chromatography on silicic acid. The availability of [U-14C]succinic semialdehyde permits the reliable assay of succinic semialdehyde dehydrogenase in crude cell extracts of lymphocytes isolated from human blood, cultured human lymphoblasts, and other tissues where 4-aminobutyric acid metabolism is known to occur.

Original languageEnglish (US)
Pages (from-to)436-437
Number of pages2
JournalAnalytical biochemistry
Volume135
Issue number2
DOIs
StatePublished - Dec 1983
Externally publishedYes

Keywords

  • GABA
  • lymphoblast
  • lymphocyte
  • monoamine oxidase
  • succinic semialdehyde
  • succinic semialdehyde dehydrogenase

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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