Enzymatic properties of mitochondria isolated from normal and vitamin B12-deficient rats

M. Abdul Matlib, Eugene P. Frenkel, Amal Mukherjee, Jerry Henslee, Paul A. Srere

Research output: Contribution to journalArticle

6 Scopus citations

Abstract

The mitochondria from livers of normal and vitamin B12-deprived animals were compared. The rate of oxygen uptake (states 3 and 4) of mitochondria from vitamin B12-deprived livers was higher than that of normal mitochondria while the respiratory control index of B12-deprived mitochondria was lower than normal. Most Krebs cycle enzyme activities were increased in the hepatic mitochondria from B12-deprived animals. Two-dimensional gel electrophoresis of mitochondrial matrix proteins also indicated that an increase in Krebs cycle enzyme quantities occurred but the increase was not general for all mitochondrial proteins.

Original languageEnglish (US)
Pages (from-to)388-395
Number of pages8
JournalArchives of Biochemistry and Biophysics
Volume197
Issue number2
DOIs
StatePublished - Oct 15 1979

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology

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