Enzymatic properties of mitochondria isolated from normal and vitamin B12-deficient rats

M. Abdul Matlib; Eugene P. Frenkel; Amal Mukherjee; Jerry Henslee; Paul A. Srere

doi:10.1016/0003-9861(79)90260-1

Enzymatic properties of mitochondria isolated from normal and vitamin B₁₂-deficient rats

M. Abdul Matlib, Eugene P. Frenkel, Amal Mukherjee, Jerry Henslee, Paul A. Srere

Research output: Contribution to journal › Article › peer-review

6 Scopus citations

Abstract

The mitochondria from livers of normal and vitamin B₁₂-deprived animals were compared. The rate of oxygen uptake (states 3 and 4) of mitochondria from vitamin B₁₂-deprived livers was higher than that of normal mitochondria while the respiratory control index of B₁₂-deprived mitochondria was lower than normal. Most Krebs cycle enzyme activities were increased in the hepatic mitochondria from B₁₂-deprived animals. Two-dimensional gel electrophoresis of mitochondrial matrix proteins also indicated that an increase in Krebs cycle enzyme quantities occurred but the increase was not general for all mitochondrial proteins.

Original language	English (US)
Pages (from-to)	388-395
Number of pages	8
Journal	Archives of Biochemistry and Biophysics
Volume	197
Issue number	2
DOIs	https://doi.org/10.1016/0003-9861(79)90260-1
State	Published - Oct 15 1979

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology

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10.1016/0003-9861(79)90260-1

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title = "Enzymatic properties of mitochondria isolated from normal and vitamin B12-deficient rats",

abstract = "The mitochondria from livers of normal and vitamin B12-deprived animals were compared. The rate of oxygen uptake (states 3 and 4) of mitochondria from vitamin B12-deprived livers was higher than that of normal mitochondria while the respiratory control index of B12-deprived mitochondria was lower than normal. Most Krebs cycle enzyme activities were increased in the hepatic mitochondria from B12-deprived animals. Two-dimensional gel electrophoresis of mitochondrial matrix proteins also indicated that an increase in Krebs cycle enzyme quantities occurred but the increase was not general for all mitochondrial proteins.",

author = "Matlib, {M. Abdul} and Frenkel, {Eugene P.} and Amal Mukherjee and Jerry Henslee and Srere, {Paul A.}",

note = "Funding Information: 1 Supported by the Veterans Administration, NSF GB 41851, and National Institutes of Health Grants AM11313 and CA23115. 2 Present address: Department of Pharmacology and Cell Biophysics, University of Cincinnati College of Medicine, Cincinnati, Ohio 45267. 3 Present address: Department of Biochemistry and Nutrition, University of North Carolina, Chapel Hill, N. C. 27514.",

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T1 - Enzymatic properties of mitochondria isolated from normal and vitamin B12-deficient rats

AU - Matlib, M. Abdul

AU - Frenkel, Eugene P.

AU - Mukherjee, Amal

AU - Henslee, Jerry

AU - Srere, Paul A.

N1 - Funding Information: 1 Supported by the Veterans Administration, NSF GB 41851, and National Institutes of Health Grants AM11313 and CA23115. 2 Present address: Department of Pharmacology and Cell Biophysics, University of Cincinnati College of Medicine, Cincinnati, Ohio 45267. 3 Present address: Department of Biochemistry and Nutrition, University of North Carolina, Chapel Hill, N. C. 27514.

PY - 1979/10/15

Y1 - 1979/10/15

N2 - The mitochondria from livers of normal and vitamin B12-deprived animals were compared. The rate of oxygen uptake (states 3 and 4) of mitochondria from vitamin B12-deprived livers was higher than that of normal mitochondria while the respiratory control index of B12-deprived mitochondria was lower than normal. Most Krebs cycle enzyme activities were increased in the hepatic mitochondria from B12-deprived animals. Two-dimensional gel electrophoresis of mitochondrial matrix proteins also indicated that an increase in Krebs cycle enzyme quantities occurred but the increase was not general for all mitochondrial proteins.

AB - The mitochondria from livers of normal and vitamin B12-deprived animals were compared. The rate of oxygen uptake (states 3 and 4) of mitochondria from vitamin B12-deprived livers was higher than that of normal mitochondria while the respiratory control index of B12-deprived mitochondria was lower than normal. Most Krebs cycle enzyme activities were increased in the hepatic mitochondria from B12-deprived animals. Two-dimensional gel electrophoresis of mitochondrial matrix proteins also indicated that an increase in Krebs cycle enzyme quantities occurred but the increase was not general for all mitochondrial proteins.

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Enzymatic properties of mitochondria isolated from normal and vitamin B₁₂-deficient rats

Abstract

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