The mitochondria from livers of normal and vitamin B12-deprived animals were compared. The rate of oxygen uptake (states 3 and 4) of mitochondria from vitamin B12-deprived livers was higher than that of normal mitochondria while the respiratory control index of B12-deprived mitochondria was lower than normal. Most Krebs cycle enzyme activities were increased in the hepatic mitochondria from B12-deprived animals. Two-dimensional gel electrophoresis of mitochondrial matrix proteins also indicated that an increase in Krebs cycle enzyme quantities occurred but the increase was not general for all mitochondrial proteins.
ASJC Scopus subject areas
- Molecular Biology