Enzymatically prepared RNAi libraries

Frank Buchholz, Ralf Kittler, Mikolaj Slabicki, Mirko Theis

Research output: Contribution to journalReview article

54 Scopus citations


Large-scale RNA interference (RNAi) screens in mammalian cells have mainly used synthetic small interfering RNA (siRNA) or short hairpin RNA (shRNA) libraries. The RNAi triggers for both of these approaches were designed with algorithm-based predictions to identify single sequences for mRNA knockdown. Alternatives to these approaches have recently been developed using enzymatic methods. Here we describe the concepts of enzymatically prepared shRNA and siRNA libraries, and discuss their strengths and limitations.

Original languageEnglish (US)
Pages (from-to)696-700
Number of pages5
JournalNature methods
Issue number9
StatePublished - Sep 1 2006


ASJC Scopus subject areas

  • Biotechnology
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Buchholz, F., Kittler, R., Slabicki, M., & Theis, M. (2006). Enzymatically prepared RNAi libraries. Nature methods, 3(9), 696-700. https://doi.org/10.1038/nmeth912