Enzyme linked immunosorbent assay

determination of anti-adenovirus antibodies in an infant population.

A. Weinberg, M. C. Fink, S. Takimoto, M. A. Ishida, M. C. Souza

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

In order to define an accurate assay for anti-adenovirus antibody detection, a recently developed ELISA was compared with IFA and CF. On 58 sera, the ELISA was more sensitive than both CF and IFA, which showed relative sensitivities of 63% and 94%, respectively. It was not possible to determine the exact specificity of the tests because of the lack of a gold standard. Furthermore, the ELISA was used to define the prevalence of adenovirus antibodies in 116 infants between 1 and 24 months old (mean 7.28). The data showed that maternal antibodies waned by the age of 5 to 6 months and that more than 80% of the children had been infected by adenoviruses by the age of 10 months.

Original languageEnglish (US)
Pages (from-to)336-340
Number of pages5
JournalRevista do Instituto de Medicina Tropical de Sao Paulo
Volume31
Issue number5
StatePublished - Sep 1989

Fingerprint

Adenoviridae
Anti-Idiotypic Antibodies
Enzyme-Linked Immunosorbent Assay
Population
Antibodies
Mothers
Serum

ASJC Scopus subject areas

  • Public Health, Environmental and Occupational Health

Cite this

Enzyme linked immunosorbent assay : determination of anti-adenovirus antibodies in an infant population. / Weinberg, A.; Fink, M. C.; Takimoto, S.; Ishida, M. A.; Souza, M. C.

In: Revista do Instituto de Medicina Tropical de Sao Paulo, Vol. 31, No. 5, 09.1989, p. 336-340.

Research output: Contribution to journalArticle

Weinberg, A. ; Fink, M. C. ; Takimoto, S. ; Ishida, M. A. ; Souza, M. C. / Enzyme linked immunosorbent assay : determination of anti-adenovirus antibodies in an infant population. In: Revista do Instituto de Medicina Tropical de Sao Paulo. 1989 ; Vol. 31, No. 5. pp. 336-340.
@article{1130469f439b4bd785491751d5ee2ba6,
title = "Enzyme linked immunosorbent assay: determination of anti-adenovirus antibodies in an infant population.",
abstract = "In order to define an accurate assay for anti-adenovirus antibody detection, a recently developed ELISA was compared with IFA and CF. On 58 sera, the ELISA was more sensitive than both CF and IFA, which showed relative sensitivities of 63{\%} and 94{\%}, respectively. It was not possible to determine the exact specificity of the tests because of the lack of a gold standard. Furthermore, the ELISA was used to define the prevalence of adenovirus antibodies in 116 infants between 1 and 24 months old (mean 7.28). The data showed that maternal antibodies waned by the age of 5 to 6 months and that more than 80{\%} of the children had been infected by adenoviruses by the age of 10 months.",
author = "A. Weinberg and Fink, {M. C.} and S. Takimoto and Ishida, {M. A.} and Souza, {M. C.}",
year = "1989",
month = "9",
language = "English (US)",
volume = "31",
pages = "336--340",
journal = "Revista do Instituto de Medicina Tropical de Sao Paulo",
issn = "0036-4665",
publisher = "Instituto de Medicina Tropical de Sao Paulo",
number = "5",

}

TY - JOUR

T1 - Enzyme linked immunosorbent assay

T2 - determination of anti-adenovirus antibodies in an infant population.

AU - Weinberg, A.

AU - Fink, M. C.

AU - Takimoto, S.

AU - Ishida, M. A.

AU - Souza, M. C.

PY - 1989/9

Y1 - 1989/9

N2 - In order to define an accurate assay for anti-adenovirus antibody detection, a recently developed ELISA was compared with IFA and CF. On 58 sera, the ELISA was more sensitive than both CF and IFA, which showed relative sensitivities of 63% and 94%, respectively. It was not possible to determine the exact specificity of the tests because of the lack of a gold standard. Furthermore, the ELISA was used to define the prevalence of adenovirus antibodies in 116 infants between 1 and 24 months old (mean 7.28). The data showed that maternal antibodies waned by the age of 5 to 6 months and that more than 80% of the children had been infected by adenoviruses by the age of 10 months.

AB - In order to define an accurate assay for anti-adenovirus antibody detection, a recently developed ELISA was compared with IFA and CF. On 58 sera, the ELISA was more sensitive than both CF and IFA, which showed relative sensitivities of 63% and 94%, respectively. It was not possible to determine the exact specificity of the tests because of the lack of a gold standard. Furthermore, the ELISA was used to define the prevalence of adenovirus antibodies in 116 infants between 1 and 24 months old (mean 7.28). The data showed that maternal antibodies waned by the age of 5 to 6 months and that more than 80% of the children had been infected by adenoviruses by the age of 10 months.

UR - http://www.scopus.com/inward/record.url?scp=0024729028&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0024729028&partnerID=8YFLogxK

M3 - Article

VL - 31

SP - 336

EP - 340

JO - Revista do Instituto de Medicina Tropical de Sao Paulo

JF - Revista do Instituto de Medicina Tropical de Sao Paulo

SN - 0036-4665

IS - 5

ER -