Enzymic removal of two oligosaccharide chains from ricin B-chain

Edward J. Wawrzynczak; Philip E. Thorpe

doi:10.1016/0014-5793(86)81490-9

Enzymic removal of two oligosaccharide chains from ricin B-chain

Edward J. Wawrzynczak, Philip E. Thorpe

Pharmacology

Research output: Contribution to journal › Article › peer-review

4 Scopus citations

Abstract

Peptide:N-glycosidase F removed both the asparagine-linked oligosaccharide chains of ricin B-chain in the absence of lactose. In the presence of lactose, which binds specifically to the B-chain, only one oligosaccharide chain was removed. Lactose also protected Ricinus communis agglutinin B-chain against the removal of one of the two susceptible oligosaccharides present in each B-chain subunit.

Original language	English (US)
Pages (from-to)	213-216
Number of pages	4
Journal	FEBS Letters
Volume	207
Issue number	2
DOIs	https://doi.org/10.1016/0014-5793(86)81490-9
State	Published - Oct 27 1986

Keywords

Agglutinin
Deglycosylation
Peptide:N-glycosidase F
Ricin
Ricin B-chain

ASJC Scopus subject areas

Biophysics
Structural Biology
Biochemistry
Molecular Biology
Genetics
Cell Biology

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10.1016/0014-5793(86)81490-9

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title = "Enzymic removal of two oligosaccharide chains from ricin B-chain",

abstract = "Peptide:N-glycosidase F removed both the asparagine-linked oligosaccharide chains of ricin B-chain in the absence of lactose. In the presence of lactose, which binds specifically to the B-chain, only one oligosaccharide chain was removed. Lactose also protected Ricinus communis agglutinin B-chain against the removal of one of the two susceptible oligosaccharides present in each B-chain subunit.",

keywords = "Agglutinin, Deglycosylation, Peptide:N-glycosidase F, Ricin, Ricin B-chain",

author = "Wawrzynczak, {Edward J.} and Thorpe, {Philip E.}",

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AU - Wawrzynczak, Edward J.

AU - Thorpe, Philip E.

PY - 1986/10/27

Y1 - 1986/10/27

N2 - Peptide:N-glycosidase F removed both the asparagine-linked oligosaccharide chains of ricin B-chain in the absence of lactose. In the presence of lactose, which binds specifically to the B-chain, only one oligosaccharide chain was removed. Lactose also protected Ricinus communis agglutinin B-chain against the removal of one of the two susceptible oligosaccharides present in each B-chain subunit.

AB - Peptide:N-glycosidase F removed both the asparagine-linked oligosaccharide chains of ricin B-chain in the absence of lactose. In the presence of lactose, which binds specifically to the B-chain, only one oligosaccharide chain was removed. Lactose also protected Ricinus communis agglutinin B-chain against the removal of one of the two susceptible oligosaccharides present in each B-chain subunit.

KW - Agglutinin

KW - Deglycosylation

KW - Peptide:N-glycosidase F

KW - Ricin

KW - Ricin B-chain

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JF - FEBS Letters

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ER -

Enzymic removal of two oligosaccharide chains from ricin B-chain

Abstract

Keywords

ASJC Scopus subject areas

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