Enzymic removal of two oligosaccharide chains from ricin B-chain

Edward J. Wawrzynczak, Philip E. Thorpe

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Peptide:N-glycosidase F removed both the asparagine-linked oligosaccharide chains of ricin B-chain in the absence of lactose. In the presence of lactose, which binds specifically to the B-chain, only one oligosaccharide chain was removed. Lactose also protected Ricinus communis agglutinin B-chain against the removal of one of the two susceptible oligosaccharides present in each B-chain subunit.

Original languageEnglish (US)
Pages (from-to)213-216
Number of pages4
JournalFEBS Letters
Volume207
Issue number2
DOIs
StatePublished - Oct 27 1986

Fingerprint

Ricin
Lactose
Oligosaccharides
Mannosyl-Glycoprotein Endo-beta-N-Acetylglucosaminidase
Asparagine

Keywords

  • Agglutinin
  • Deglycosylation
  • Peptide:N-glycosidase F
  • Ricin
  • Ricin B-chain

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Enzymic removal of two oligosaccharide chains from ricin B-chain. / Wawrzynczak, Edward J.; Thorpe, Philip E.

In: FEBS Letters, Vol. 207, No. 2, 27.10.1986, p. 213-216.

Research output: Contribution to journalArticle

Wawrzynczak, Edward J. ; Thorpe, Philip E. / Enzymic removal of two oligosaccharide chains from ricin B-chain. In: FEBS Letters. 1986 ; Vol. 207, No. 2. pp. 213-216.
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