Enzymic removal of two oligosaccharide chains from ricin B-chain

Edward J. Wawrzynczak, Philip E. Thorpe

Research output: Contribution to journalArticlepeer-review

4 Scopus citations

Abstract

Peptide:N-glycosidase F removed both the asparagine-linked oligosaccharide chains of ricin B-chain in the absence of lactose. In the presence of lactose, which binds specifically to the B-chain, only one oligosaccharide chain was removed. Lactose also protected Ricinus communis agglutinin B-chain against the removal of one of the two susceptible oligosaccharides present in each B-chain subunit.

Original languageEnglish (US)
Pages (from-to)213-216
Number of pages4
JournalFEBS Letters
Volume207
Issue number2
DOIs
StatePublished - Oct 27 1986

Keywords

  • Agglutinin
  • Deglycosylation
  • Peptide:N-glycosidase F
  • Ricin
  • Ricin B-chain

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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