EphB forward signaling controls directional branch extension and arborization required for dorsal-ventral retinotopic mapping

Robert Hindges, Todd McLaughlin, Nicolas Genoud, Mark Henkemeyer, Dennis D M O'Leary

Research output: Contribution to journalArticle

251 Scopus citations

Abstract

We report that EphB receptors direct unique axonal behaviors required for mapping the dorsal-ventral (D-V) retinal axis along the lateral-medial (L-M) axis of the superior colliculus (SC). EphBs are expressed in a D-V gradient, ephrin-B1 in a L-M gradient in SC, and ephrin-B3 at its midline. EphBs and ephrin-Bs are expressed in countergradients in retina and SC. Developmental analyses reveal that retinal axons lack D-V ordering along the L-M axis, but directionally extend branches along it to establish ordered arbors. Directed branch extension is disrupted in EphB2; EphB3-deficient mice resulting in lateral ectopic arbors. Mice with kinase-inactive EphB2 have similar D-V mapping defects indicating that forward signaling dominates over reverse signaling. Our data suggest that branches of EphB expressing axons are attracted medially by ephrin-B1, and provide molecular mechanisms for D-V mapping in visual centers.

Original languageEnglish (US)
Pages (from-to)475-487
Number of pages13
JournalNeuron
Volume35
Issue number3
DOIs
StatePublished - Aug 1 2002

ASJC Scopus subject areas

  • Neuroscience(all)

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