EphB forward signaling controls directional branch extension and arborization required for dorsal-ventral retinotopic mapping

Robert Hindges, Todd McLaughlin, Nicolas Genoud, Mark Henkemeyer, Dennis D M O'Leary

Research output: Contribution to journalArticle

248 Citations (Scopus)

Abstract

We report that EphB receptors direct unique axonal behaviors required for mapping the dorsal-ventral (D-V) retinal axis along the lateral-medial (L-M) axis of the superior colliculus (SC). EphBs are expressed in a D-V gradient, ephrin-B1 in a L-M gradient in SC, and ephrin-B3 at its midline. EphBs and ephrin-Bs are expressed in countergradients in retina and SC. Developmental analyses reveal that retinal axons lack D-V ordering along the L-M axis, but directionally extend branches along it to establish ordered arbors. Directed branch extension is disrupted in EphB2; EphB3-deficient mice resulting in lateral ectopic arbors. Mice with kinase-inactive EphB2 have similar D-V mapping defects indicating that forward signaling dominates over reverse signaling. Our data suggest that branches of EphB expressing axons are attracted medially by ephrin-B1, and provide molecular mechanisms for D-V mapping in visual centers.

Original languageEnglish (US)
Pages (from-to)475-487
Number of pages13
JournalNeuron
Volume35
Issue number3
DOIs
StatePublished - Aug 1 2002

Fingerprint

Superior Colliculi
Ephrin-B1
Ephrin-B3
Axons
Eph Family Receptors
Ephrins
Retina
Phosphotransferases

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

EphB forward signaling controls directional branch extension and arborization required for dorsal-ventral retinotopic mapping. / Hindges, Robert; McLaughlin, Todd; Genoud, Nicolas; Henkemeyer, Mark; O'Leary, Dennis D M.

In: Neuron, Vol. 35, No. 3, 01.08.2002, p. 475-487.

Research output: Contribution to journalArticle

Hindges, Robert ; McLaughlin, Todd ; Genoud, Nicolas ; Henkemeyer, Mark ; O'Leary, Dennis D M. / EphB forward signaling controls directional branch extension and arborization required for dorsal-ventral retinotopic mapping. In: Neuron. 2002 ; Vol. 35, No. 3. pp. 475-487.
@article{eae56657936e49d998a363320a865f79,
title = "EphB forward signaling controls directional branch extension and arborization required for dorsal-ventral retinotopic mapping",
abstract = "We report that EphB receptors direct unique axonal behaviors required for mapping the dorsal-ventral (D-V) retinal axis along the lateral-medial (L-M) axis of the superior colliculus (SC). EphBs are expressed in a D-V gradient, ephrin-B1 in a L-M gradient in SC, and ephrin-B3 at its midline. EphBs and ephrin-Bs are expressed in countergradients in retina and SC. Developmental analyses reveal that retinal axons lack D-V ordering along the L-M axis, but directionally extend branches along it to establish ordered arbors. Directed branch extension is disrupted in EphB2; EphB3-deficient mice resulting in lateral ectopic arbors. Mice with kinase-inactive EphB2 have similar D-V mapping defects indicating that forward signaling dominates over reverse signaling. Our data suggest that branches of EphB expressing axons are attracted medially by ephrin-B1, and provide molecular mechanisms for D-V mapping in visual centers.",
author = "Robert Hindges and Todd McLaughlin and Nicolas Genoud and Mark Henkemeyer and O'Leary, {Dennis D M}",
year = "2002",
month = "8",
day = "1",
doi = "10.1016/S0896-6273(02)00799-7",
language = "English (US)",
volume = "35",
pages = "475--487",
journal = "Neuron",
issn = "0896-6273",
publisher = "Cell Press",
number = "3",

}

TY - JOUR

T1 - EphB forward signaling controls directional branch extension and arborization required for dorsal-ventral retinotopic mapping

AU - Hindges, Robert

AU - McLaughlin, Todd

AU - Genoud, Nicolas

AU - Henkemeyer, Mark

AU - O'Leary, Dennis D M

PY - 2002/8/1

Y1 - 2002/8/1

N2 - We report that EphB receptors direct unique axonal behaviors required for mapping the dorsal-ventral (D-V) retinal axis along the lateral-medial (L-M) axis of the superior colliculus (SC). EphBs are expressed in a D-V gradient, ephrin-B1 in a L-M gradient in SC, and ephrin-B3 at its midline. EphBs and ephrin-Bs are expressed in countergradients in retina and SC. Developmental analyses reveal that retinal axons lack D-V ordering along the L-M axis, but directionally extend branches along it to establish ordered arbors. Directed branch extension is disrupted in EphB2; EphB3-deficient mice resulting in lateral ectopic arbors. Mice with kinase-inactive EphB2 have similar D-V mapping defects indicating that forward signaling dominates over reverse signaling. Our data suggest that branches of EphB expressing axons are attracted medially by ephrin-B1, and provide molecular mechanisms for D-V mapping in visual centers.

AB - We report that EphB receptors direct unique axonal behaviors required for mapping the dorsal-ventral (D-V) retinal axis along the lateral-medial (L-M) axis of the superior colliculus (SC). EphBs are expressed in a D-V gradient, ephrin-B1 in a L-M gradient in SC, and ephrin-B3 at its midline. EphBs and ephrin-Bs are expressed in countergradients in retina and SC. Developmental analyses reveal that retinal axons lack D-V ordering along the L-M axis, but directionally extend branches along it to establish ordered arbors. Directed branch extension is disrupted in EphB2; EphB3-deficient mice resulting in lateral ectopic arbors. Mice with kinase-inactive EphB2 have similar D-V mapping defects indicating that forward signaling dominates over reverse signaling. Our data suggest that branches of EphB expressing axons are attracted medially by ephrin-B1, and provide molecular mechanisms for D-V mapping in visual centers.

UR - http://www.scopus.com/inward/record.url?scp=0036685537&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0036685537&partnerID=8YFLogxK

U2 - 10.1016/S0896-6273(02)00799-7

DO - 10.1016/S0896-6273(02)00799-7

M3 - Article

C2 - 12165470

AN - SCOPUS:0036685537

VL - 35

SP - 475

EP - 487

JO - Neuron

JF - Neuron

SN - 0896-6273

IS - 3

ER -