EphB regulates L1 phosphorylation during retinocollicular mapping

Jinxia Dai, Jasbir S. Dalal, Sonal Thakar, Mark Henkemeyer, Vance P. Lemmon, Jill S. Harunaga, Monika C. Schlatter, Mona Buhusi, Patricia F. Maness

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Interaction of the cell adhesion molecule L1 with the cytoskeletal adaptor ankyrin is essential for topographic mapping of retinal ganglion cell (RGC) axons to synaptic targets in the superior colliculus (SC). Mice mutated in the L1 ankyrin-binding motif (FIGQY 1229H) display abnormal mapping of RGC axons along the mediolateral axis of the SC, resembling mouse mutant phenotypes in EphB receptor tyrosine kinases. To investigate whether L1 functionally interacts with EphBs, we investigated the role of EphB kinases in phosphorylating L1 using a phospho-specific antibody to the tyrosine phosphorylated FIGQY 1229 motif. EphB2, but not an EphB2 kinase dead mutant, induced tyrosine phosphorylation of L1 at FIGQY 1229 and perturbed ankyrin recruitment to the membrane in L1-transfected HEK293 cells. Src family kinases mediated L1 phosphorylation at FIGQY 1229 by EphB2. Other EphB receptors that regulate medial-lateral retinocollicular mapping, EphB1 and EphB3, also mediated phosphorylation of L1 at FIGQY 1229. Tyrosine 1176 in the cytoplasmic domain of L1, which regulates AP2/clathrin-mediated endocytosis and axonal trafficking, was not phosphorylated by EphB2. Accordingly mutation of Tyr 1176 to Ala in L1-Y 1176A knock-in mice resulted in normal retinocollicular mapping of ventral RGC axons. Immunostaining of the mouse SC during retinotopic mapping showed that L1 colocalized with phospho-FIGQY in RGC axons in retinorecipient layers. Immunoblotting of SC lysates confirmed that L1 was phosphorylated at FIGQY 1229 in wild type but not L1-FIGQY 1229H (L1Y 1229H) mutant SC, and that L1 phosphorylation was decreased in the EphB2/B3 mutant SC. Inhibition of ankyrin binding in L1Y 1229H mutant RGCs resulted in increased neurite outgrowth compared to WT RGCs in retinal explant cultures, suggesting that L1-ankyrin binding serves to constrain RGC axon growth. These findings are consistent with a model in which EphB kinases phosphorylate L1 at FIGQY 1229 in retinal axons to modulate L1-ankyrin binding important for mediolateral retinocollicular topography.

Original languageEnglish (US)
Pages (from-to)201-210
Number of pages10
JournalMolecular and Cellular Neuroscience
Volume50
Issue number2
DOIs
StatePublished - Jun 2012

Fingerprint

Ankyrins
Superior Colliculi
Retinal Ganglion Cells
Axons
Phosphorylation
Eph Family Receptors
Tyrosine
Phosphotransferases
Neural Cell Adhesion Molecule L1
Phospho-Specific Antibodies
Clathrin
src-Family Kinases
HEK293 Cells
Endocytosis
Immunoblotting
Protein-Tyrosine Kinases
Phenotype
Mutation
Membranes
Growth

Keywords

  • Ankyrin
  • EphB receptor
  • FIGQY domain
  • L1
  • Retinocollicular mapping
  • Tyrosine phosphorylation

ASJC Scopus subject areas

  • Molecular Biology
  • Cellular and Molecular Neuroscience
  • Cell Biology

Cite this

Dai, J., Dalal, J. S., Thakar, S., Henkemeyer, M., Lemmon, V. P., Harunaga, J. S., ... Maness, P. F. (2012). EphB regulates L1 phosphorylation during retinocollicular mapping. Molecular and Cellular Neuroscience, 50(2), 201-210. https://doi.org/10.1016/j.mcn.2012.05.001

EphB regulates L1 phosphorylation during retinocollicular mapping. / Dai, Jinxia; Dalal, Jasbir S.; Thakar, Sonal; Henkemeyer, Mark; Lemmon, Vance P.; Harunaga, Jill S.; Schlatter, Monika C.; Buhusi, Mona; Maness, Patricia F.

In: Molecular and Cellular Neuroscience, Vol. 50, No. 2, 06.2012, p. 201-210.

Research output: Contribution to journalArticle

Dai, J, Dalal, JS, Thakar, S, Henkemeyer, M, Lemmon, VP, Harunaga, JS, Schlatter, MC, Buhusi, M & Maness, PF 2012, 'EphB regulates L1 phosphorylation during retinocollicular mapping', Molecular and Cellular Neuroscience, vol. 50, no. 2, pp. 201-210. https://doi.org/10.1016/j.mcn.2012.05.001
Dai, Jinxia ; Dalal, Jasbir S. ; Thakar, Sonal ; Henkemeyer, Mark ; Lemmon, Vance P. ; Harunaga, Jill S. ; Schlatter, Monika C. ; Buhusi, Mona ; Maness, Patricia F. / EphB regulates L1 phosphorylation during retinocollicular mapping. In: Molecular and Cellular Neuroscience. 2012 ; Vol. 50, No. 2. pp. 201-210.
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abstract = "Interaction of the cell adhesion molecule L1 with the cytoskeletal adaptor ankyrin is essential for topographic mapping of retinal ganglion cell (RGC) axons to synaptic targets in the superior colliculus (SC). Mice mutated in the L1 ankyrin-binding motif (FIGQY 1229H) display abnormal mapping of RGC axons along the mediolateral axis of the SC, resembling mouse mutant phenotypes in EphB receptor tyrosine kinases. To investigate whether L1 functionally interacts with EphBs, we investigated the role of EphB kinases in phosphorylating L1 using a phospho-specific antibody to the tyrosine phosphorylated FIGQY 1229 motif. EphB2, but not an EphB2 kinase dead mutant, induced tyrosine phosphorylation of L1 at FIGQY 1229 and perturbed ankyrin recruitment to the membrane in L1-transfected HEK293 cells. Src family kinases mediated L1 phosphorylation at FIGQY 1229 by EphB2. Other EphB receptors that regulate medial-lateral retinocollicular mapping, EphB1 and EphB3, also mediated phosphorylation of L1 at FIGQY 1229. Tyrosine 1176 in the cytoplasmic domain of L1, which regulates AP2/clathrin-mediated endocytosis and axonal trafficking, was not phosphorylated by EphB2. Accordingly mutation of Tyr 1176 to Ala in L1-Y 1176A knock-in mice resulted in normal retinocollicular mapping of ventral RGC axons. Immunostaining of the mouse SC during retinotopic mapping showed that L1 colocalized with phospho-FIGQY in RGC axons in retinorecipient layers. Immunoblotting of SC lysates confirmed that L1 was phosphorylated at FIGQY 1229 in wild type but not L1-FIGQY 1229H (L1Y 1229H) mutant SC, and that L1 phosphorylation was decreased in the EphB2/B3 mutant SC. Inhibition of ankyrin binding in L1Y 1229H mutant RGCs resulted in increased neurite outgrowth compared to WT RGCs in retinal explant cultures, suggesting that L1-ankyrin binding serves to constrain RGC axon growth. These findings are consistent with a model in which EphB kinases phosphorylate L1 at FIGQY 1229 in retinal axons to modulate L1-ankyrin binding important for mediolateral retinocollicular topography.",
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AU - Dalal, Jasbir S.

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AU - Lemmon, Vance P.

AU - Harunaga, Jill S.

AU - Schlatter, Monika C.

AU - Buhusi, Mona

AU - Maness, Patricia F.

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N2 - Interaction of the cell adhesion molecule L1 with the cytoskeletal adaptor ankyrin is essential for topographic mapping of retinal ganglion cell (RGC) axons to synaptic targets in the superior colliculus (SC). Mice mutated in the L1 ankyrin-binding motif (FIGQY 1229H) display abnormal mapping of RGC axons along the mediolateral axis of the SC, resembling mouse mutant phenotypes in EphB receptor tyrosine kinases. To investigate whether L1 functionally interacts with EphBs, we investigated the role of EphB kinases in phosphorylating L1 using a phospho-specific antibody to the tyrosine phosphorylated FIGQY 1229 motif. EphB2, but not an EphB2 kinase dead mutant, induced tyrosine phosphorylation of L1 at FIGQY 1229 and perturbed ankyrin recruitment to the membrane in L1-transfected HEK293 cells. Src family kinases mediated L1 phosphorylation at FIGQY 1229 by EphB2. Other EphB receptors that regulate medial-lateral retinocollicular mapping, EphB1 and EphB3, also mediated phosphorylation of L1 at FIGQY 1229. Tyrosine 1176 in the cytoplasmic domain of L1, which regulates AP2/clathrin-mediated endocytosis and axonal trafficking, was not phosphorylated by EphB2. Accordingly mutation of Tyr 1176 to Ala in L1-Y 1176A knock-in mice resulted in normal retinocollicular mapping of ventral RGC axons. Immunostaining of the mouse SC during retinotopic mapping showed that L1 colocalized with phospho-FIGQY in RGC axons in retinorecipient layers. Immunoblotting of SC lysates confirmed that L1 was phosphorylated at FIGQY 1229 in wild type but not L1-FIGQY 1229H (L1Y 1229H) mutant SC, and that L1 phosphorylation was decreased in the EphB2/B3 mutant SC. Inhibition of ankyrin binding in L1Y 1229H mutant RGCs resulted in increased neurite outgrowth compared to WT RGCs in retinal explant cultures, suggesting that L1-ankyrin binding serves to constrain RGC axon growth. These findings are consistent with a model in which EphB kinases phosphorylate L1 at FIGQY 1229 in retinal axons to modulate L1-ankyrin binding important for mediolateral retinocollicular topography.

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KW - EphB receptor

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